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Isolation of Nebulin from Rabbit Skeletal Muscle and Its Interaction with Actin
Joint Authors
Watanabe, Atsushi
Bao, Yulong
Kimura, Sumiko
Sasano, Kouhei
Asano, Masato
Maruyama, Koscak
Hanashima, Akira
Chitose, Ryo
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Nebulin is about 800 kDa filamentous protein that binds the entire thin filament of vertebrate skeletal muscle sarcomeres.
Nebulin cannot be isolated from muscle except in a completely denatured form by direct solubilization of myofibrils with SDS because nebulin is hardly soluble under salt conditions.
In the present study, nebulin was solubilized by a salt solution containing 1 M urea and purified by DEAE-Toyopearl column chromatography via 4 M urea elution.
Rotary-shadowed images of nebulin showed entangled knit-like particles, about 20 nm in diameter.
The purified nebulin bound to actin filaments to form loose bundles.
Nebulin was confirmed to bind actin, α-actinin, β-actinin, and tropomodulin, but not troponin or tropomyosin.
The data shows that full-length nebulin can be also obtained in a functional and presumably native form, verified by data from experiments using recombinant subfragments.
American Psychological Association (APA)
Chitose, Ryo& Watanabe, Atsushi& Asano, Masato& Hanashima, Akira& Sasano, Kouhei& Bao, Yulong…[et al.]. 2010. Isolation of Nebulin from Rabbit Skeletal Muscle and Its Interaction with Actin. BioMed Research International،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-988749
Modern Language Association (MLA)
Chitose, Ryo…[et al.]. Isolation of Nebulin from Rabbit Skeletal Muscle and Its Interaction with Actin. BioMed Research International No. 2010 (Dec. 2010), pp.1-8.
https://search.emarefa.net/detail/BIM-988749
American Medical Association (AMA)
Chitose, Ryo& Watanabe, Atsushi& Asano, Masato& Hanashima, Akira& Sasano, Kouhei& Bao, Yulong…[et al.]. Isolation of Nebulin from Rabbit Skeletal Muscle and Its Interaction with Actin. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-988749
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988749