The Masticatory Contractile Load Induced Expression and Activation of Akt1PKBα in Muscle Fibers at the Myotendinous Junction within Muscle-Tendon-Bone Unit
Joint Authors
Bloch, Wilhelm
Raab, Wolfgang H.-M.
Korkmaz, Yüksel
Moghbeli, Mehrnoush
Addicks, Klaus
Klinz, Franz J.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-04-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The cell specific detection of enzyme activation in response to the physiological contractile load within muscle-tendon-bone unit is essential for understanding of the mechanical forces transmission from muscle cells via tendon to the bone.
The hypothesis that the physiological mechanical loading regulates activation of Akt1/PKBα at Thr308 and at Ser473 in muscle fibers within muscle-tendon-bone unit was tested using quantitative immunohistochemistry, confocal double fluorescence analysis, and immunoblot analysis.
In comparison to the staining intensities in peripheral regions of the muscle fibers, Akt1/PKBα was detected with a higher staining intensity in muscle fibers at the myotendinous junction (MTJ) areas.
In muscle fibers at the MTJ areas, Akt1/PKBα is dually phosphorylated at Thr308 and Ser473.
The immunohistochemical results were confirmed by immunoblot analysis.
We conclude that contractile load generated by masticatory muscles induces local domain-dependent expression of Akt1/PKBα as well as activation by dually phosphorylation at Thr308 and Ser473 in muscle fibers at the MTJ areas within muscle-tendon-bone unit.
American Psychological Association (APA)
Korkmaz, Yüksel& Klinz, Franz J.& Moghbeli, Mehrnoush& Addicks, Klaus& Raab, Wolfgang H.-M.& Bloch, Wilhelm. 2010. The Masticatory Contractile Load Induced Expression and Activation of Akt1PKBα in Muscle Fibers at the Myotendinous Junction within Muscle-Tendon-Bone Unit. BioMed Research International،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-988783
Modern Language Association (MLA)
Korkmaz, Yüksel…[et al.]. The Masticatory Contractile Load Induced Expression and Activation of Akt1PKBα in Muscle Fibers at the Myotendinous Junction within Muscle-Tendon-Bone Unit. BioMed Research International No. 2010 (Dec. 2010), pp.1-8.
https://search.emarefa.net/detail/BIM-988783
American Medical Association (AMA)
Korkmaz, Yüksel& Klinz, Franz J.& Moghbeli, Mehrnoush& Addicks, Klaus& Raab, Wolfgang H.-M.& Bloch, Wilhelm. The Masticatory Contractile Load Induced Expression and Activation of Akt1PKBα in Muscle Fibers at the Myotendinous Junction within Muscle-Tendon-Bone Unit. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-988783
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988783