Bioimaging of Nucleolin Aptamer-Containing 5-(N-benzylcarboxyamide)‎-2′-deoxyuridine More Capable of Specific Binding to Targets in Cancer Cells

Joint Authors

Kim, Soonhag
Lee, Dong Soo
Kang, Hyungu
Lee, Jung Hwan
Ryu, Sung Ho
Lee, Kyue Yim

Source

BioMed Research International

Issue

Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2010-02-28

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Medicine

Abstract EN

Chemically modified nucleotides have been developed and applied into SELEX procedure to find a novel type of aptamers to fit with targets of interest.

In this study, we directly performed chemical modification of 5-(N-benzylcarboxyamide)-2′-deoxyuridine (called 5-BzdU) in the AS1411 aptamer, which binds to the nucleolin protein expressed in cancer cells.

Forty-seven compounds of AS1411-containing Cy3-labeled 5-BzdU (called Cy3-(5-BzdU)-modified-AS1411) were synthesized by randomly substituting thymidines one to twelve in AS1411 with Cy3-labeled 5-BzdU.

Both statistically quantified fluorescence measurements and confocal imaging analysis demonstrated at least three potential compounds of interest: number 12, 29 and 41 that significantly increased the targeting affinity to cancer cells but no significant activity from normal healthy cells.

These results suggest that the position and number of substituents in AS1411 are critical parameters to improve the aptamer function.

In this study, we demonstrated that chemical modification of the existing aptamers enhanced the binding and targeting affinity to targets of interest without additional SELEX procedures.

American Psychological Association (APA)

Lee, Kyue Yim& Kang, Hyungu& Ryu, Sung Ho& Lee, Dong Soo& Lee, Jung Hwan& Kim, Soonhag. 2010. Bioimaging of Nucleolin Aptamer-Containing 5-(N-benzylcarboxyamide)-2′-deoxyuridine More Capable of Specific Binding to Targets in Cancer Cells. BioMed Research International،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-988787

Modern Language Association (MLA)

Lee, Kyue Yim…[et al.]. Bioimaging of Nucleolin Aptamer-Containing 5-(N-benzylcarboxyamide)-2′-deoxyuridine More Capable of Specific Binding to Targets in Cancer Cells. BioMed Research International No. 2010 (Dec. 2010), pp.1-9.
https://search.emarefa.net/detail/BIM-988787

American Medical Association (AMA)

Lee, Kyue Yim& Kang, Hyungu& Ryu, Sung Ho& Lee, Dong Soo& Lee, Jung Hwan& Kim, Soonhag. Bioimaging of Nucleolin Aptamer-Containing 5-(N-benzylcarboxyamide)-2′-deoxyuridine More Capable of Specific Binding to Targets in Cancer Cells. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-988787

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-988787