Statistical Analysis of Variation in the Human Plasma Proteome
Joint Authors
Choi, Megan W.
Corzett, Todd H.
Walsworth, Vicki L.
Turteltaub, Kenneth W.
McCutchen-Maloney, Sandra L.
Fodor, Imola K.
Chromy, Brett A.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-12-06
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Quantifying the variation in the human plasma proteome is an essential prerequisite for disease-specific biomarker detection.
We report here on the longitudinal and individual variation in human plasma characterized by two-dimensional difference gel electrophoresis (2-D DIGE) using plasma samples from eleven healthy subjects collected three times over a two week period.
Fixed-effects modeling was used to remove dye and gel variability.
Mixed-effects modeling was then used to quantitate the sources of proteomic variation.
The subject-to-subject variation represented the largest variance component, while the time-within-subject variation was comparable to the experimental variation found in a previous technical variability study where one human plasma sample was processed eight times in parallel and each was then analyzed by 2-D DIGE in triplicate.
Here, 21 protein spots had larger than 50% CV, suggesting that these proteins may not be appropriate as biomarkers and should be carefully scrutinized in future studies.
Seventy-eight protein spots showing differential protein levels between different individuals or individual collections were identified by mass spectrometry and further characterized using hierarchical clustering.
The results present a first step toward understanding the complexity of longitudinal and individual variation in the human plasma proteome, and provide a baseline for improved biomarker discovery.
American Psychological Association (APA)
Corzett, Todd H.& Fodor, Imola K.& Choi, Megan W.& Walsworth, Vicki L.& Turteltaub, Kenneth W.& McCutchen-Maloney, Sandra L.…[et al.]. 2009. Statistical Analysis of Variation in the Human Plasma Proteome. BioMed Research International،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-988828
Modern Language Association (MLA)
Corzett, Todd H.…[et al.]. Statistical Analysis of Variation in the Human Plasma Proteome. BioMed Research International No. 2010 (Dec. 2010), pp.1-12.
https://search.emarefa.net/detail/BIM-988828
American Medical Association (AMA)
Corzett, Todd H.& Fodor, Imola K.& Choi, Megan W.& Walsworth, Vicki L.& Turteltaub, Kenneth W.& McCutchen-Maloney, Sandra L.…[et al.]. Statistical Analysis of Variation in the Human Plasma Proteome. BioMed Research International. 2009. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-988828
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988828