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Expression and Immunogenicity of the Mycobacterial Ag85BESAT-6 Antigens Produced in Transgenic Plants by Elastin-Like Peptide Fusion Strategy
Joint Authors
Chevaleyre, Claire
Zanello, Galliano
Diogon, Marie
Mockey, Michael
Conrad, Udo
Berri, Mustapha
Rodrigues, Valérie
Garzon, Edwin
Salmon, Henri
Frutos, Roger
Bruel, Timothée
Dedieu, Laurence
Floss, Doreen Manuela
Brosson, Damien
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-07
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
This study explored a novel system combining plant-based production and the elastin-like peptide (ELP) fusion strategy to produce vaccinal antigens against tuberculosis.
Transgenic tobacco plants expressing the mycobacterial antigens Ag85B and ESAT-6 fused to ELP (TBAg-ELP) were generated.
Purified TBAg-ELP was obtained by the highly efficient, cost-effective, inverse transition cycling (ICT) method and tested in mice.
Furthermore, safety and immunogenicity of the crude tobacco leaf extracts were assessed in piglets.
Antibodies recognizing mycobacterial antigens were produced in mice and piglets.
A T-cell immune response able to recognize the native mycobacterial antigens was detected in mice.
These findings showed that the native Ag85B and ESAT-6 mycobacterial B- and T-cell epitopes were conserved in the plant-expressed TBAg-ELP.
This study presents the first results of an efficient plant-expression system, relying on the elastin-like peptide fusion strategy, to produce a safe and immunogenic mycobacterial Ag85B-ESAT-6 fusion protein as a potential vaccine candidate against tuberculosis.
American Psychological Association (APA)
Floss, Doreen Manuela& Mockey, Michael& Zanello, Galliano& Brosson, Damien& Diogon, Marie& Frutos, Roger…[et al.]. 2010. Expression and Immunogenicity of the Mycobacterial Ag85BESAT-6 Antigens Produced in Transgenic Plants by Elastin-Like Peptide Fusion Strategy. BioMed Research International،Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-988835
Modern Language Association (MLA)
Floss, Doreen Manuela…[et al.]. Expression and Immunogenicity of the Mycobacterial Ag85BESAT-6 Antigens Produced in Transgenic Plants by Elastin-Like Peptide Fusion Strategy. BioMed Research International No. 2010 (Dec. 2010), pp.1-14.
https://search.emarefa.net/detail/BIM-988835
American Medical Association (AMA)
Floss, Doreen Manuela& Mockey, Michael& Zanello, Galliano& Brosson, Damien& Diogon, Marie& Frutos, Roger…[et al.]. Expression and Immunogenicity of the Mycobacterial Ag85BESAT-6 Antigens Produced in Transgenic Plants by Elastin-Like Peptide Fusion Strategy. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-988835
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988835