Hunting for Serine 276-Phosphorylated p65
Joint Authors
Gerlo, Sarah
Kolmus, Krzysztof
Spooren, Anneleen
Haegeman, Guy
Vermeulen, Linda
Van Wesemael, Karlien
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-12-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The transcription factor nuclear factor kappaB (NF-κB) is one of the central mediators of inflammatory gene expression.
Several posttranslational modifications of NF-κB, regulating its transactivation ability, have been described.
Especially phosphorylation of the NF-κB subunit p65 has been investigated in depth and several commercial phosphospecific antibodies, targeting selected p65 residues, are available.
One of the p65 residues, that is subject to phosphorylation by protein kinase A (PKA) as well as by mitogen-stimulated kinase-1 (MSK-1), is the serine at position 276.
Here, we have performed a detailed analysis of the performance of the most commonly used commercial anti-P-p65 Ser276 antibodies.
Our findings indicate that at least three widely used anti-P-p65 Ser276 antibodies do not detect p65 in vivo via Western Blot, but instead crossreact with PKA-regulated proteins.
As PKA is one of the main kinases responsible for phosphorylation of p65 at Ser276, this observation warrants cautious interpretation of data generated using the tested antibodies.
American Psychological Association (APA)
Spooren, Anneleen& Kolmus, Krzysztof& Vermeulen, Linda& Van Wesemael, Karlien& Haegeman, Guy& Gerlo, Sarah. 2009. Hunting for Serine 276-Phosphorylated p65. BioMed Research International،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-988836
Modern Language Association (MLA)
Spooren, Anneleen…[et al.]. Hunting for Serine 276-Phosphorylated p65. BioMed Research International No. 2010 (Dec. 2010), pp.1-9.
https://search.emarefa.net/detail/BIM-988836
American Medical Association (AMA)
Spooren, Anneleen& Kolmus, Krzysztof& Vermeulen, Linda& Van Wesemael, Karlien& Haegeman, Guy& Gerlo, Sarah. Hunting for Serine 276-Phosphorylated p65. BioMed Research International. 2009. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-988836
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988836