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Gene Transfer into the Lung by Nanoparticle Dextran-SperminePlasmid DNA Complexes
Joint Authors
Domb, Abraham J.
Hosseinkhani, Mohsen
Hosseinkhani, Hossein
Rosli, Rozita
Wendy-Yeo, Wai Yeng
Rahman, Sabariah A.
Ramasamy, Rajesh
Abdullah, Syahril
Masrawa, Ehab
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A novel cationic polymer, dextran-spermine (D-SPM), has been found to mediate gene expression in a wide variety of cell lines and in vivo through systemic delivery.
Here, we extended the observations by determining the optimal conditions for gene expression of D-SPM/plasmid DNA (D-SPM/pDNA) in cell lines and in the lungs of BALB/c mice via instillation delivery.
In vitro studies showed that D-SPM could partially protect pDNA from degradation by nuclease and exhibited optimal gene transfer efficiency at D-SPM to pDNA weight-mixing ratio of 12.
In the lungs of mice, the levels of gene expression generated by D-SPM/pDNA are highly dependent on the weight-mixing ratio of D-SPM to pDNA, amount of pDNA in the complex, and the assay time postdelivery.
Readministration of the complex at day 1 following the first dosing showed no significant effect on the retention and duration of gene expression.
The study also showed that there was a clear trend of increasing size of the complexes as the amount of pDNA was increased, where the sizes of the D-SPM/pDNA complexes were within the nanometer range.
American Psychological Association (APA)
Abdullah, Syahril& Wendy-Yeo, Wai Yeng& Hosseinkhani, Hossein& Hosseinkhani, Mohsen& Masrawa, Ehab& Ramasamy, Rajesh…[et al.]. 2010. Gene Transfer into the Lung by Nanoparticle Dextran-SperminePlasmid DNA Complexes. BioMed Research International،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-988842
Modern Language Association (MLA)
Abdullah, Syahril…[et al.]. Gene Transfer into the Lung by Nanoparticle Dextran-SperminePlasmid DNA Complexes. BioMed Research International No. 2010 (Dec. 2010), pp.1-10.
https://search.emarefa.net/detail/BIM-988842
American Medical Association (AMA)
Abdullah, Syahril& Wendy-Yeo, Wai Yeng& Hosseinkhani, Hossein& Hosseinkhani, Mohsen& Masrawa, Ehab& Ramasamy, Rajesh…[et al.]. Gene Transfer into the Lung by Nanoparticle Dextran-SperminePlasmid DNA Complexes. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-988842
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988842