In Vivo Bioassay of Recombinant Human Growth Hormone Synthesized in B. mori Pupae
Joint Authors
Lan, Hanglian
Quan, Yanping
Zhang, Yaozhou
Lv, Zhengbing
Wu, Xiangfu
Chen, Jianqing
Liu, Yingshuo
Chen, Qin
Sheng, Qing
Liu, Yue
Chen, Jian
Wang, Dan
Nie, Zuoming
Zhen, Qingliang
Yu, Wei
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-04
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The human growth hormone (hGH) has been expressed in prokaryotic expression system with low bioactivity previously.
Then the effective B.
mori baculovirus system was employed to express hGH identical to mature hGH successfully in larvae, but the expression level was still limited.
In this work, the hGH was expressed in B.
mori pupae by baculovirus system.
Quantification of recombinant hGH protein (BmrhGH) showed that the expression of BmrhGH reached the level of approximately 890 μg/mL pupae supernatant solution, which was five times more than the level using larvae.
Furthermore, Animals were gavaged with BmrhGH at the dose of 4.5 mg/rat.day, and the body weight gain (BWG) of treated group had a significant difference (P<.01) compared with the control group.
The other two parameters of liver weight and epiphyseal width were also found to be different between the two groups (P<.05).
The results suggested that BmrhGH might be used as a protein drug by oral administration.
American Psychological Association (APA)
Lan, Hanglian& Nie, Zuoming& Liu, Yue& Lv, Zhengbing& Liu, Yingshuo& Quan, Yanping…[et al.]. 2010. In Vivo Bioassay of Recombinant Human Growth Hormone Synthesized in B. mori Pupae. BioMed Research International،Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-988854
Modern Language Association (MLA)
Lan, Hanglian…[et al.]. In Vivo Bioassay of Recombinant Human Growth Hormone Synthesized in B. mori Pupae. BioMed Research International No. 2010 (Dec. 2010), pp.1-6.
https://search.emarefa.net/detail/BIM-988854
American Medical Association (AMA)
Lan, Hanglian& Nie, Zuoming& Liu, Yue& Lv, Zhengbing& Liu, Yingshuo& Quan, Yanping…[et al.]. In Vivo Bioassay of Recombinant Human Growth Hormone Synthesized in B. mori Pupae. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-988854
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988854