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High Throughput T Epitope Mapping and Vaccine Development
Joint Authors
Ivaldi, Federico
Moretti, Paolo
Manca, Fabrizio
Li Pira, Giuseppina
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-25
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Mapping of antigenic peptide sequences from proteins of relevant pathogens recognized by T helper (Th) and by cytolytic T lymphocytes (CTL) is crucial for vaccine development.
In fact, mapping of T-cell epitopes provides useful information for the design of peptide-based vaccines and of peptide libraries to monitor specific cellular immunity in protected individuals, patients and vaccinees.
Nevertheless, epitope mapping is a challenging task.
In fact, large panels of overlapping peptides need to be tested with lymphocytes to identify the sequences that induce a T-cell response.
Since numerous peptide panels from antigenic proteins are to be screened, lymphocytes available from human subjects are a limiting factor.
To overcome this limitation, high throughput (HTP) approaches based on miniaturization and automation of T-cell assays are needed.
Here we consider the most recent applications of the HTP approach to T epitope mapping.
The alternative or complementary use of in silico prediction and experimental epitope definition is discussed in the context of the recent literature.
The currently used methods are described with special reference to the possibility of applying the HTP concept to make epitope mapping an easier procedure in terms of time, workload, reagents, cells and overall cost.
American Psychological Association (APA)
Li Pira, Giuseppina& Ivaldi, Federico& Moretti, Paolo& Manca, Fabrizio. 2010. High Throughput T Epitope Mapping and Vaccine Development. BioMed Research International،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-988863
Modern Language Association (MLA)
Li Pira, Giuseppina…[et al.]. High Throughput T Epitope Mapping and Vaccine Development. BioMed Research International No. 2010 (Dec. 2010), pp.1-12.
https://search.emarefa.net/detail/BIM-988863
American Medical Association (AMA)
Li Pira, Giuseppina& Ivaldi, Federico& Moretti, Paolo& Manca, Fabrizio. High Throughput T Epitope Mapping and Vaccine Development. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-988863
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988863