Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy
Joint Authors
Junghans, Richard P.
Wang, Yawen
Lo, Agnes Shuk-Yee
Ma, Qiangzhong
Gomes, Erica M.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-06
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The successful ex vivo expansion of a large numbers of T cells is a prerequisite for adoptive immunotherapy.
In this study, we found that cell density had important effects on the process of expansion of T cells in vitro.
Resting T cells were activated to expand at high cell density but failed to be activated at low cell density.
Activated T cells (ATCs) expanded rapidly at high cell density but underwent apoptosis at low cell density.
Our studies indicated that low-cell-density related ATC death is mediated by oxidative stress.
Antioxidants N-acetylcysteine, catalase, and albumin suppressed elevated reactive oxygen species (ROS) levels in low-density cultures and protected ATCs from apoptosis.
The viability of ATCs at low density was preserved by conditioned medium from high-density cultures of ATCs in which the autocrine survival factor was identified as catalase.
We also found that costimulatory signal CD28 increases T cell activation at lower cell density, paralleled by an increase in catalase secretion.
Our findings highlight the importance of cell density in T cell activation, proliferation, survival and apoptosis and support the importance of maintaining T cells at high density for their successful expansion in vitro.
American Psychological Association (APA)
Ma, Qiangzhong& Wang, Yawen& Lo, Agnes Shuk-Yee& Gomes, Erica M.& Junghans, Richard P.. 2010. Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy. BioMed Research International،Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-988888
Modern Language Association (MLA)
Ma, Qiangzhong…[et al.]. Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy. BioMed Research International No. 2010 (Dec. 2010), pp.1-13.
https://search.emarefa.net/detail/BIM-988888
American Medical Association (AMA)
Ma, Qiangzhong& Wang, Yawen& Lo, Agnes Shuk-Yee& Gomes, Erica M.& Junghans, Richard P.. Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-988888
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988888