Construction and Characterization of Insect Cell-Derived Influenza VLP: Cell Binding, Fusion, and EGFP Incorporation
Joint Authors
Wei, Hung-Ju
Lin, Chung-Hung
Chang, Chung-Chieh
Wei, Ting-Shyang
Wu, Suh-Chin
Chang, Ding-Kwo
Pan, Yi-Shin
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-14
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We have constructed virus-like particles (VLPs) harboring hemagglutinin (HA), neuraminidase (NA), matrix protein 1 (M1) ,and proton channel protein (M2) using baculovirus as a vector in the SF9 insect cell.
The size of the expressed VLP was estimated to be ~100 nm by light scattering experiment and transmission electron microscopy.
Recognition of HA on the VLP surface by the HA2-specific monoclonal antibody IIF4 at acidic pH, as probed by surface plasmon resonance, indicated the pH-induced structural rearrangement of HA.
Uptake of the particle by A549 mediated by HA-sialylose receptor interaction was visualized by the fluorescent-labeled VLP.
The HA-promoted cell-virus fusion activity was illustrated by fluorescence imaging on the Jurkat cells incubated with rhodamine-loaded VLP performed at fusogenic pH.
Furthermore, the green fluorescence protein (GFP) was fused to NA to produce VLP with a pH-sensitive probe, expanding the use of VLP as an antigen carrier and a tool for viral tracking.
American Psychological Association (APA)
Pan, Yi-Shin& Wei, Hung-Ju& Chang, Chung-Chieh& Lin, Chung-Hung& Wei, Ting-Shyang& Wu, Suh-Chin…[et al.]. 2010. Construction and Characterization of Insect Cell-Derived Influenza VLP: Cell Binding, Fusion, and EGFP Incorporation. BioMed Research International،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-988946
Modern Language Association (MLA)
Pan, Yi-Shin…[et al.]. Construction and Characterization of Insect Cell-Derived Influenza VLP: Cell Binding, Fusion, and EGFP Incorporation. BioMed Research International No. 2010 (Dec. 2010), pp.1-11.
https://search.emarefa.net/detail/BIM-988946
American Medical Association (AMA)
Pan, Yi-Shin& Wei, Hung-Ju& Chang, Chung-Chieh& Lin, Chung-Hung& Wei, Ting-Shyang& Wu, Suh-Chin…[et al.]. Construction and Characterization of Insect Cell-Derived Influenza VLP: Cell Binding, Fusion, and EGFP Incorporation. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-988946
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988946