The Adenovirus Type 3 Dodecahedron's RGD Loop Comprisesan HSPG Binding Site That Influences Integrin Binding
Joint Authors
Fenel, D.
Lortat-Jacob, H.
Schoehn, G.
Gout, E.
Fender, P.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-02-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Human type 3 adenovirus dodecahedron (a virus like particle made of twelve penton bases) features the ability to enter cells through Heparan Sulphate Proteoglycans (HSPGs) and integrins interaction and is used as a versatile vector to deliver DNA or proteins.
Cryo-EM reconstruction of the pseudoviral particle with Heparan Sulphate (HS) oligosaccharide shows an extradensity on the RGD loop.
A set of mutants was designed to study the respective roles of the RGD sequence (RGE mutant) and of a basic sequence located just downstream.
Results showed that the RGE mutant binding to the HS deficient CHO-2241 cells was abolished and unexpectedly, mutation of the basic sequence (KQKR to AQAS) dramatically decreased integrin recognition by the viral pseudoparticle.
This basic sequence is thus involved in integrin docking, showing a close interplay between HSPGs and integrin receptors.
American Psychological Association (APA)
Gout, E.& Schoehn, G.& Fenel, D.& Lortat-Jacob, H.& Fender, P.. 2010. The Adenovirus Type 3 Dodecahedron's RGD Loop Comprisesan HSPG Binding Site That Influences Integrin Binding. BioMed Research International،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-988978
Modern Language Association (MLA)
Gout, E.…[et al.]. The Adenovirus Type 3 Dodecahedron's RGD Loop Comprisesan HSPG Binding Site That Influences Integrin Binding. BioMed Research International No. 2010 (Dec. 2010), pp.1-7.
https://search.emarefa.net/detail/BIM-988978
American Medical Association (AMA)
Gout, E.& Schoehn, G.& Fenel, D.& Lortat-Jacob, H.& Fender, P.. The Adenovirus Type 3 Dodecahedron's RGD Loop Comprisesan HSPG Binding Site That Influences Integrin Binding. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-988978
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988978