Increasing a Robust Antigen-Specific Cytotoxic T Lymphocyte Response by FMDV DNA Vaccination with IL-9 Expressing Construct
Joint Authors
Wang, Bin
He, Xiaodan
Kang, Youmin
Zou, Qiang
Liu, Hu
Wu, Bing
Xu, Hanqian
Zhang, Yizhi
Jin, Jin
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Various chemokines and cytokines as adjuvants can be used to improve efficacy of DNA vaccination.
In this study, we sought to investigate if a DNA construct expressing IL-9 (designed as proV-IL9) as a molecular adjuvant enhance antigen specific immune responses elicited by the pcD-VP1 DNA vaccination.
Mice immunized with pcD-VP1 combined with proV-IL9 developed a strong humoral response.
In addition, the coinoculation induced significant higher level of antigen-specific cell proliferation and cytotoxic response.
This agreed well with higher expression level of IFN-γ and perforin in CD8+ T cells, but not with IL-17 in these T cells.
The results indicate that IL-9 induces the development of IFN-γ-producing CD8+ T cells (Tc1), but not the IL-17-producing CD8+ T cells (Tc17).
Up-regulated expressions of BCL-2 and BCL-XL were exhibited in these Tc1 cells, suggesting that IL-9 may trigger antiapoptosis mechanism in these cells.
Together, these results demonstrated that IL-9 used as molecular adjuvant could enhance the immunogenicity of DNA vaccination, in augmenting humoral and cellular responses and particularly promoting Tc1 activations.
Thus, the IL-9 may be utilized as a potent Tc1 adjuvant for DNA vaccines.
American Psychological Association (APA)
Zou, Qiang& Wu, Bing& He, Xiaodan& Zhang, Yizhi& Kang, Youmin& Jin, Jin…[et al.]. 2010. Increasing a Robust Antigen-Specific Cytotoxic T Lymphocyte Response by FMDV DNA Vaccination with IL-9 Expressing Construct. BioMed Research International،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-988993
Modern Language Association (MLA)
Zou, Qiang…[et al.]. Increasing a Robust Antigen-Specific Cytotoxic T Lymphocyte Response by FMDV DNA Vaccination with IL-9 Expressing Construct. BioMed Research International No. 2010 (Dec. 2010), pp.1-8.
https://search.emarefa.net/detail/BIM-988993
American Medical Association (AMA)
Zou, Qiang& Wu, Bing& He, Xiaodan& Zhang, Yizhi& Kang, Youmin& Jin, Jin…[et al.]. Increasing a Robust Antigen-Specific Cytotoxic T Lymphocyte Response by FMDV DNA Vaccination with IL-9 Expressing Construct. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-988993
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988993