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Dynamic Metabolic Flux Analysis Demonstrated on Cultures Where the Limiting Substrate Is Changed from Carbon to Nitrogen and Vice Versa
Joint Authors
Maertens, Jo
Soetaert, Wim
Vanrolleghem, Peter A.
Van Horen, Ellen
Vandamme, Erick
Beauprez, Joeri
Lequeux, Gaspard
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-13
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
The main requirement for metabolic flux analysis (MFA) is that the cells are in a pseudo-steady state, that there is no accumulation or depletion of intracellular metabolites.
In the past, the applications of MFA were limited to the analysis of continuous cultures.
This contribution introduces the concept of dynamic MFA and extends MFA so that it is applicable to transient cultures.
Time series of concentration measurements are transformed into flux values.
This transformation involves differentiation, which typically increases the noisiness of the data.
Therefore, a noise-reducing step is needed.
In this work, polynomial smoothing was used.
As a test case, dynamic MFA is applied on Escherichia coli cultivations shifting from carbon limitation to nitrogen limitation and vice versa.
After switching the limiting substrate from N to C, a lag phase was observed accompanied with an increase in maintenance energy requirement.
This lag phase did not occur in the C- to N-limitation case.
American Psychological Association (APA)
Lequeux, Gaspard& Beauprez, Joeri& Maertens, Jo& Van Horen, Ellen& Soetaert, Wim& Vandamme, Erick…[et al.]. 2010. Dynamic Metabolic Flux Analysis Demonstrated on Cultures Where the Limiting Substrate Is Changed from Carbon to Nitrogen and Vice Versa. BioMed Research International،Vol. 2010, no. 2010, pp.1-19.
https://search.emarefa.net/detail/BIM-989041
Modern Language Association (MLA)
Lequeux, Gaspard…[et al.]. Dynamic Metabolic Flux Analysis Demonstrated on Cultures Where the Limiting Substrate Is Changed from Carbon to Nitrogen and Vice Versa. BioMed Research International No. 2010 (Dec. 2010), pp.1-19.
https://search.emarefa.net/detail/BIM-989041
American Medical Association (AMA)
Lequeux, Gaspard& Beauprez, Joeri& Maertens, Jo& Van Horen, Ellen& Soetaert, Wim& Vandamme, Erick…[et al.]. Dynamic Metabolic Flux Analysis Demonstrated on Cultures Where the Limiting Substrate Is Changed from Carbon to Nitrogen and Vice Versa. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-19.
https://search.emarefa.net/detail/BIM-989041
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989041