Ordered Assembly of the Adhesive and Electrochemical Connections within Newly Formed Intercalated Disks in Primary Cultures of Adult Rat Cardiomyocytes
Joint Authors
Isom, Lori L.
Meshinchi, Sasha
Geisler, Sarah B.
Delmar, Mario
Green, Kathleen J.
Russell, Mark W.
Martens, Jeffrey R.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-12
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The intercalated disk (ID) is a complex structure that electromechanically couples adjoining cardiac myocytes into a functional syncitium.
The integrity of the disk is essential for normal cardiac function, but how the diverse elements are assembled into a fully integrated structure is not well understood.
In this study, we examined the assembly of new IDs in primary cultures of adult rat cardiac myocytes.
From 2 to 5 days after dissociation, the cells flatten and spread, establishing new cell-cell contacts in a manner that recapitulates the in vivo processes that occur during heart development and myocardial remodeling.
As cells make contact with their neighbors, transmembrane adhesion proteins localize along the line of apposition, concentrating at the sites of membrane attachment of the terminal sarcomeres.
Cx43 gap junctions and ankyrin-G, an essential cytoskeletal component of voltage gated sodium channel complexes, were secondarily recruited to membrane domains involved in cell-cell contacts.
The consistent order of the assembly process suggests that there are specific scaffolding requirements for integration of the mechanical and electrochemical elements of the disk.
Defining the relationships that are the foundation of disk assembly has important implications for understanding the mechanical dysfunction and cardiac arrhythmias that accompany alterations of ID architecture.
American Psychological Association (APA)
Geisler, Sarah B.& Green, Kathleen J.& Isom, Lori L.& Meshinchi, Sasha& Martens, Jeffrey R.& Delmar, Mario…[et al.]. 2010. Ordered Assembly of the Adhesive and Electrochemical Connections within Newly Formed Intercalated Disks in Primary Cultures of Adult Rat Cardiomyocytes. BioMed Research International،Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-989043
Modern Language Association (MLA)
Geisler, Sarah B.…[et al.]. Ordered Assembly of the Adhesive and Electrochemical Connections within Newly Formed Intercalated Disks in Primary Cultures of Adult Rat Cardiomyocytes. BioMed Research International No. 2010 (Dec. 2010), pp.1-14.
https://search.emarefa.net/detail/BIM-989043
American Medical Association (AMA)
Geisler, Sarah B.& Green, Kathleen J.& Isom, Lori L.& Meshinchi, Sasha& Martens, Jeffrey R.& Delmar, Mario…[et al.]. Ordered Assembly of the Adhesive and Electrochemical Connections within Newly Formed Intercalated Disks in Primary Cultures of Adult Rat Cardiomyocytes. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-989043
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989043