Endoplasmic Reticulum Stress-Mediated Apoptosis Involved in Indirect Recognition Pathway Blockade Induces Long-Term Heart Allograft Survival
Joint Authors
Gu, Xiaodong
Xiang, Jianbin
Chen, Zongyou
Qian, Shiguang
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Implementation of dendritic cell- (DC-) based therapies in organ transplantation can reduce dependency on nonspecific immunosuppression.
Despite extensive research, mechanisms of equipped DCs inducing transplant tolerance remain incomplete.
Here, we applied RNA interference technique to inhibit CD80 and CD86 expression in host bone marrow-derived DCs.
This approach could specifically and effectively knock down CD80 and CD86 expression.
T cells primed by these DCs inhibited allogeneic responses.
Administration of recipient DCs loaded with alloantigen after CD80 and CD86 blockade prolonged cardiac allograft survival.
We also found a higher percentage of apoptotic T cells in lymph tissues and grafts than that detected in control group.
In addition, these T cells expressed high expression of GRP78 than controls, indicating activation of unfolded protein responses.
Upregulation of CHOP expression among these cells suggested that the endoplasmic reticulum stress (ERS) response switched to a proapoptotic response.
Our results indicated that ERS-induced apoptosis may be involved in allogeneic T-cell apoptosis, and the ERS-mediated apoptosis pathway may be a novel target in clinical prevention and therapy of allograft rejection.
American Psychological Association (APA)
Xiang, Jianbin& Gu, Xiaodong& Qian, Shiguang& Chen, Zongyou. 2010. Endoplasmic Reticulum Stress-Mediated Apoptosis Involved in Indirect Recognition Pathway Blockade Induces Long-Term Heart Allograft Survival. BioMed Research International،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-989075
Modern Language Association (MLA)
Xiang, Jianbin…[et al.]. Endoplasmic Reticulum Stress-Mediated Apoptosis Involved in Indirect Recognition Pathway Blockade Induces Long-Term Heart Allograft Survival. BioMed Research International No. 2010 (Dec. 2010), pp.1-10.
https://search.emarefa.net/detail/BIM-989075
American Medical Association (AMA)
Xiang, Jianbin& Gu, Xiaodong& Qian, Shiguang& Chen, Zongyou. Endoplasmic Reticulum Stress-Mediated Apoptosis Involved in Indirect Recognition Pathway Blockade Induces Long-Term Heart Allograft Survival. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-989075
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989075