Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody Production
Joint Authors
Li, Yi-jing
Ma, Guang-peng
Ge, Jun-wei
Qiao, Xin-yuan
Liu, Li-wei
Tang, Li-jie
Liu, Min
Li, Gui-wei
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The objective of this study to design a delivery system resistant to the gastrointestinal environment for oral vaccine against porcine rotavirus.
Lactococcus lactis NZ9000 was transformed with segments of vP4 of the porcine rotavirus inserted into the pNZ8112 surface-expression vector, and a recombinant L.
lactis expressing VP4 protein was constructed.
An approximately 27 kDa VP4 protein was confirmed by SDS-PAGE , Western blot and immunostaining analysis.
BALB/c mice were immunized orally with VP4-expression recombinant L.
lactis and cellular, mucosal and systemic humoral immune responses were examined.
Specific anti-VP4 secretory IgA and IgG were found in feces, ophthalmic and vaginal washes and in serum.
The induced antibodies demonstrated neutralizing effects on porcine rotavirus infection on MA104 cells.
Our findings suggest that oral immunization with VP4-expressing L.
lactis induced both specific local and systemic humoral and cellular immune responses in mice.
American Psychological Association (APA)
Li, Yi-jing& Ma, Guang-peng& Li, Gui-wei& Qiao, Xin-yuan& Ge, Jun-wei& Tang, Li-jie…[et al.]. 2010. Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody Production. BioMed Research International،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-989079
Modern Language Association (MLA)
Li, Yi-jing…[et al.]. Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody Production. BioMed Research International No. 2010 (Dec. 2010), pp.1-9.
https://search.emarefa.net/detail/BIM-989079
American Medical Association (AMA)
Li, Yi-jing& Ma, Guang-peng& Li, Gui-wei& Qiao, Xin-yuan& Ge, Jun-wei& Tang, Li-jie…[et al.]. Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody Production. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-989079
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989079