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Aberrant Methylation of Thrombospondin-1 and Its Association with Reduced Expression in Gastric Cardia Adenocarcinoma
Joint Authors
Yang, Zhibin
Chen, Zhifeng
Guo, Wei
Kuang, Gang
Dong, Zhiming
He, Ming
Guo, Jianwen
Guo, Yanli
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-02-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Aim.
Investigate the promoter methylation of the Thrombospondin-1 (TSP1) gene in gastric cardia adenocarcinoma (GCA).
Methods.
MSP approach, immunohistochemistry method, and RT-PCR were used respectively to examine the promoter methylation of TSP1, its protein and mRNA expression in tumors and corresponding normal tissues.
The expression and concentration of TGF-β1 were examined respectively by immunohistochemistry and ELISA method.
The status of T cell immunity was examined by Flow cytometry analysis.
Results.
TSP1 was methylated in 34/96 (35.4%) tumor specimens, which was significantly higher than that in corresponding normal tissues (P<.001).
Protein and mRNA expression of TSP1 in GCA tumor tissues were reduced significantly and were associated with TSP1 methylation.
The protein expression of TGF-β1 was significantly higher in tumor tissues (P<.001) and was associated with TNM stage and histological differentiation.
The concentration of active and total TGF-β1 did not show significant difference between the GCA patients with hypermethylation of TSP1 and without methylation of TSP1 (P>.05).
The function of T cell immunity was significantly different between the GCA patients with hypermethylation of TSP1 and without methylation of TSP1.
Conclusions.
Epigenetic silencing of TSP1 gene by promoter hypermethylation may play an important role in GCA.
American Psychological Association (APA)
Guo, Wei& Dong, Zhiming& He, Ming& Guo, Yanli& Guo, Jianwen& Chen, Zhifeng…[et al.]. 2010. Aberrant Methylation of Thrombospondin-1 and Its Association with Reduced Expression in Gastric Cardia Adenocarcinoma. BioMed Research International،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-989088
Modern Language Association (MLA)
Guo, Wei…[et al.]. Aberrant Methylation of Thrombospondin-1 and Its Association with Reduced Expression in Gastric Cardia Adenocarcinoma. BioMed Research International No. 2010 (Dec. 2010), pp.1-10.
https://search.emarefa.net/detail/BIM-989088
American Medical Association (AMA)
Guo, Wei& Dong, Zhiming& He, Ming& Guo, Yanli& Guo, Jianwen& Chen, Zhifeng…[et al.]. Aberrant Methylation of Thrombospondin-1 and Its Association with Reduced Expression in Gastric Cardia Adenocarcinoma. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-989088
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989088