Nonviral Gene Delivery to Mesenchymal Stem Cells Using Cationic Liposomes for Gene and Cell Therapy
Joint Authors
Ribeiro, S. C.
Boura, J. S.
Mendes, R. D.
da Silva, C. L.
Madeira, C.
Cabral, J. M. S.
Aires-Barros, M. R.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-13
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Mesenchymal stem cells (MSCs) hold a great promise for application in several therapies due to their unique biological characteristics.
In order to harness their full potential in cell-or gene-based therapies it might be advantageous to enhance some of their features through gene delivery strategies.
Accordingly, we are interested in developing an efficient and safe methodology to genetically engineer human bone marrow MSC (BM MSC), enhancing their therapeutic efficacy in Regenerative Medicine.
The plasmid DNA delivery was optimized using a cationic liposome-based reagent.
Transfection efficiencies ranged from ~2% to ~35%, resulting from using a Lipid/DNA ratio of 1.25 with a transgene expression of 7 days.
Importantly, the number of plasmid copies in different cell passages was quantified for the first time and ~20,000 plasmid copies/cell were obtained independently of cell passage.
As transfected MSC have shown high viabilities (>90%) and recoveries (>52%) while maintaining their multipotency, this might be an advantageous transfection strategy when the goal is to express a therapeutic gene in a safe and transient way.
American Psychological Association (APA)
Madeira, C.& Mendes, R. D.& Ribeiro, S. C.& Boura, J. S.& Aires-Barros, M. R.& da Silva, C. L.…[et al.]. 2010. Nonviral Gene Delivery to Mesenchymal Stem Cells Using Cationic Liposomes for Gene and Cell Therapy. BioMed Research International،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-989096
Modern Language Association (MLA)
Madeira, C.…[et al.]. Nonviral Gene Delivery to Mesenchymal Stem Cells Using Cationic Liposomes for Gene and Cell Therapy. BioMed Research International No. 2010 (Dec. 2010), pp.1-12.
https://search.emarefa.net/detail/BIM-989096
American Medical Association (AMA)
Madeira, C.& Mendes, R. D.& Ribeiro, S. C.& Boura, J. S.& Aires-Barros, M. R.& da Silva, C. L.…[et al.]. Nonviral Gene Delivery to Mesenchymal Stem Cells Using Cationic Liposomes for Gene and Cell Therapy. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-989096
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989096