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Multiple-Locus Variable-Number Tandem Repeat Analysis of Vibrio cholerae in Comparison with Pulsed Field Gel Electrophoresis and Virulotyping
Joint Authors
Thong, Kwai-Lin
Chua, Kek Heng
Teh, Cindy Shuan Ju
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Molecular analysis of Malaysian Vibrio cholerae was carried out using a multiple-locus variable-number tandem repeat analysis (MLVA) assay based on 7 loci of V.
cholerae.
The discriminatory ability of the assay was compared with pulsed-field gel electrophoresis (PFGE) using 43 Malaysian V.
cholerae isolated from various sources.
In addition, the virulotypes of the strains were determined.
Based on MLVA, 38 allelic profiles were obtained (F=0.63) while PFGE generated 35 pulsotypes (F=0.71).
Simpson's index of diversity for different VNTR loci ranged from 0.59 to 0.92.
The combined loci increased the discriminatory index to 0.99 which was comparable with PFGE (D=0.99).
Most of the environmental non-O1/non-O139 strains harbored rtxA, rstR, toxR, and hlyA only, and the virulotype of this serogroup was significantly different (P<.01) from clinical/environmental O1 and environmental O139 strains.
In conclusion, the MLVA assay developed in this study was a useful genotyping tool with comparable discriminatory power with PFGE.
In addition, the combination of the two approaches can further distinguish the strains from different sources and geographical regions of isolation.
American Psychological Association (APA)
Teh, Cindy Shuan Ju& Chua, Kek Heng& Thong, Kwai-Lin. 2010. Multiple-Locus Variable-Number Tandem Repeat Analysis of Vibrio cholerae in Comparison with Pulsed Field Gel Electrophoresis and Virulotyping. BioMed Research International،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-989131
Modern Language Association (MLA)
Teh, Cindy Shuan Ju…[et al.]. Multiple-Locus Variable-Number Tandem Repeat Analysis of Vibrio cholerae in Comparison with Pulsed Field Gel Electrophoresis and Virulotyping. BioMed Research International No. 2010 (Dec. 2010), pp.1-7.
https://search.emarefa.net/detail/BIM-989131
American Medical Association (AMA)
Teh, Cindy Shuan Ju& Chua, Kek Heng& Thong, Kwai-Lin. Multiple-Locus Variable-Number Tandem Repeat Analysis of Vibrio cholerae in Comparison with Pulsed Field Gel Electrophoresis and Virulotyping. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-989131
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989131