RNAi-Based Strategies for Cyclooxygenase-2 Inhibition in Cancer

Joint Authors

Griffoni, Cristiana
Lazzarini, Giorgia
Valerii, Maria Chiara
Spisni, Enzo
Strillacci, Antonio
Tomasi, Vittorio

Source

BioMed Research International

Issue

Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2010-05-17

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Medicine

Abstract EN

Cyclooxygenase-2 (COX-2) enzyme has been involved in the tumorigenesis and in the progression of colorectal cancer (CRC).

The use of traditional nonsteroidal anti-inflammatory drugs (NSAIDs) or selective COX-2 inhibitors has been proposed for the prevention and the treatment of this relevant neoplastic disease.

In the light of an innovative alternative to these pharmacological approaches, we review here the possible strategies to achieve a strong and selective inhibition of COX-2 enzyme by using the mechanism of RNA Interference (RNAi) targeted against its mRNA.

Anti-COX-2 siRNA molecules (siCOX-2) can be generated in CRC cells from short hairpin RNA (shRNA) precursors, delivered in vitro by a retroviral expression system, and induce a significant and stable silencing of overexpressed COX-2 in human colon cancer cells.

As a safer alternative to viral approach, nonpathogenic bacteria (E.

coli) can be engineered to invade eukaryotic cells and to generate siCOX-2 molecules in cancer cells.

Moreover, the involvement of miRNAs in COX-2 posttranscriptional regulation opens up the possibility to exploit an endogenous silencing mechanism to knockdown overexpressed COX-2.

Thus, these recent strategies disclose new challenging perspectives for the development of clinically compatible siRNA or miRNA capable of selectively inhibiting COX-2 enzyme.

American Psychological Association (APA)

Strillacci, Antonio& Griffoni, Cristiana& Valerii, Maria Chiara& Lazzarini, Giorgia& Tomasi, Vittorio& Spisni, Enzo. 2010. RNAi-Based Strategies for Cyclooxygenase-2 Inhibition in Cancer. BioMed Research International،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-989136

Modern Language Association (MLA)

Strillacci, Antonio…[et al.]. RNAi-Based Strategies for Cyclooxygenase-2 Inhibition in Cancer. BioMed Research International No. 2010 (Dec. 2010), pp.1-11.
https://search.emarefa.net/detail/BIM-989136

American Medical Association (AMA)

Strillacci, Antonio& Griffoni, Cristiana& Valerii, Maria Chiara& Lazzarini, Giorgia& Tomasi, Vittorio& Spisni, Enzo. RNAi-Based Strategies for Cyclooxygenase-2 Inhibition in Cancer. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-989136

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-989136