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Prime-Boost Vaccination Using Chemokine-Fused gp120 DNA and HIV Envelope Peptides Activates Both Immediate and Long-Term Memory Cellular Responses in Rhesus Macaques
Joint Authors
Cha, Soung-chul
He, Hong
Qin, Hong
Buchl, Stephanie
Nehete, Pramod N.
Sastry, Jagannadha K.
Kwak, Larry W.
Nehete, Bharti
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
HIV vaccine candidates with improved immunogenicity and induction of mucosal T-cell immunity are needed.
A prime-boost strategy using a novel HIV glycoprotein 120 DNA vaccine was employed to immunize rhesus macaques.
The DNA vaccine encoded a chimeric gp120 protein in fusion with monocyte chemoattractant protein-3, which was hypothesized to improve the ability of antigen-presenting cells to capture viral antigen through chemokine receptor-mediated endocytosis.
DNA vaccination induced virus-reactive T cells in peripheral blood, detectable by T cell proliferation, INFγ ELISPOT and sustained IL-6 production, without humoral responses.
With a peptide-cocktail vaccine containing a set of conserved polypeptides of HIV-1 envelope protein, given by nasogastric administration, primed T-cell immunity was significantly boosted.
Surprisingly, long-term and peptide-specific mucosal memory T-cell immunity was detected in both vaccinated macaques after one year.
Therefore, data from this investigation offer proof-of-principle for potential effectiveness of the prime-boost strategy with a chemokine-fused gp120 DNA and warrant further testing in the nonhuman primate models for developing as a potential HIV vaccine candidate in humans.
American Psychological Association (APA)
Qin, Hong& Nehete, Pramod N.& He, Hong& Nehete, Bharti& Buchl, Stephanie& Cha, Soung-chul…[et al.]. 2010. Prime-Boost Vaccination Using Chemokine-Fused gp120 DNA and HIV Envelope Peptides Activates Both Immediate and Long-Term Memory Cellular Responses in Rhesus Macaques. BioMed Research International،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-989161
Modern Language Association (MLA)
Qin, Hong…[et al.]. Prime-Boost Vaccination Using Chemokine-Fused gp120 DNA and HIV Envelope Peptides Activates Both Immediate and Long-Term Memory Cellular Responses in Rhesus Macaques. BioMed Research International No. 2010 (Dec. 2010), pp.1-7.
https://search.emarefa.net/detail/BIM-989161
American Medical Association (AMA)
Qin, Hong& Nehete, Pramod N.& He, Hong& Nehete, Bharti& Buchl, Stephanie& Cha, Soung-chul…[et al.]. Prime-Boost Vaccination Using Chemokine-Fused gp120 DNA and HIV Envelope Peptides Activates Both Immediate and Long-Term Memory Cellular Responses in Rhesus Macaques. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-989161
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989161