Release of Glycoprotein (GP1) from the Tegumental Surface of Taenia solium by Phospholipase C from Clostridium perfringens Suggests a Novel Protein-Anchor to Membranes
Joint Authors
Willms, Kaethe
Landa-Piedra, Abraham
Laclette, Juan Pedro
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-01-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
In order to explore how molecules are linked to the membrane surface in larval Taenia solium, whole cysticerci were incubated in the presence of phospholipase C from Clostridium perfringens (PLC).
Released material was collected and analyzed in polyacrylamide gels with sodium dodecyl sulfate.
Two major bands with apparent molecular weights of 180 and 43 kDa were observed.
Western blot of released material and localization assays in cysticerci tissue sections using antibodies against five known surface glycoproteins of T.
solium cysticerci indicated that only one, previously called GP1, was released.
Similar localization studies using the lectins wheat-germ-agglutinin and Concanavalin A showed that N-acetyl-D-glucosamine, N-acetylneuraminic, sialic acid, αmethyl-D-mannoside, D-manose/glucose, and N-acetyl-D-glucosamine residues are abundantly present on the surface.
On the other hand, we find that treatment with PLC releases molecules from the surface; they do not reveal Cross Reacting Determinant (CRD), suggesting a novel anchor to the membrane for the glycoprotein GP1.
American Psychological Association (APA)
Landa-Piedra, Abraham& Willms, Kaethe& Laclette, Juan Pedro. 2010. Release of Glycoprotein (GP1) from the Tegumental Surface of Taenia solium by Phospholipase C from Clostridium perfringens Suggests a Novel Protein-Anchor to Membranes. BioMed Research International،Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-989181
Modern Language Association (MLA)
Landa-Piedra, Abraham…[et al.]. Release of Glycoprotein (GP1) from the Tegumental Surface of Taenia solium by Phospholipase C from Clostridium perfringens Suggests a Novel Protein-Anchor to Membranes. BioMed Research International No. 2010 (Dec. 2010), pp.1-6.
https://search.emarefa.net/detail/BIM-989181
American Medical Association (AMA)
Landa-Piedra, Abraham& Willms, Kaethe& Laclette, Juan Pedro. Release of Glycoprotein (GP1) from the Tegumental Surface of Taenia solium by Phospholipase C from Clostridium perfringens Suggests a Novel Protein-Anchor to Membranes. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-989181
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989181