Preliminary Characterization of the Transcriptional Response of the Porcine Intestinal Cell Line IPEC-J2 to Enterotoxigenic Escherichia coli, Escherichia coli, and E. coli Lipopolysaccharide
Joint Authors
Geens, Marisa M.
Niewold, Theo A.
Source
International Journal of Genomics
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
IPEC-J2, a promising in vitro model system, is not well characterized especially on the transcriptional level, in contrast to human counterparts.
The aim of this study was to characterize the gene expression in IPEC-J2 cells when coincubated with enterotoxigenic Escherichia coli (ETEC), nonpathogenic E.
coli, and E.
coli endotoxin.
Apical infection of polarized IPEC-J2 monolayers caused a time-dependent decrease in transepithelial electrical resistance (TEER).
Microarray analysis showed up-regulation of interleukins when IPEC-J2 were cocultured with E.
coli strains this has so far never been measured in this cell line.
Highest IL8 expression was found with the ETEC strain possessing the F4 fimbrium, suggesting IPEC-J2 cells to be F4 receptor positive, confirmed in a brush border membrane adhesion assay.
It is concluded that the innate immune responses to pathogens and LPS makes the IPEC-J2 cell line a suitable model for research on intestinal host pathogen interaction.
American Psychological Association (APA)
Geens, Marisa M.& Niewold, Theo A.. 2010. Preliminary Characterization of the Transcriptional Response of the Porcine Intestinal Cell Line IPEC-J2 to Enterotoxigenic Escherichia coli, Escherichia coli, and E. coli Lipopolysaccharide. International Journal of Genomics،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-989232
Modern Language Association (MLA)
Geens, Marisa M.& Niewold, Theo A.. Preliminary Characterization of the Transcriptional Response of the Porcine Intestinal Cell Line IPEC-J2 to Enterotoxigenic Escherichia coli, Escherichia coli, and E. coli Lipopolysaccharide. International Journal of Genomics No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-989232
American Medical Association (AMA)
Geens, Marisa M.& Niewold, Theo A.. Preliminary Characterization of the Transcriptional Response of the Porcine Intestinal Cell Line IPEC-J2 to Enterotoxigenic Escherichia coli, Escherichia coli, and E. coli Lipopolysaccharide. International Journal of Genomics. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-989232
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989232