Generation and Analysis of Expressed Sequence Tags from Olea europaea L.
Joint Authors
Oruç, Fatma
Ozdemir Ozgenturk, Nehir
Sezerman, Ugur
Toksoz, Feriha
Un, Cemal
Vural Korkut, Senay
Kuçukural, Alper
Source
International Journal of Genomics
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Olive (Olea europaea L.) is an important source of edible oil which was originated in Near-East region.
In this study, two cDNA libraries were constructed from young olive leaves and immature olive fruits for generation of ESTs to discover the novel genes and search the function of unknown genes of olive.
The randomly selected 3840 colonies were sequenced for EST collection from both libraries.
Readable 2228 sequences for olive leaf and 1506 sequences for olive fruit were assembled into 205 and 69 contigs, respectively, whereas 2478 were singletons.
Putative functions of all 2752 differentially expressed unique sequences were designated by gene homology based on BLAST and annotated using BLAST2GO.
While 1339 ESTs show no homology to the database, 2024 ESTs have homology (under 80%) with hypothetical proteins, putative proteins, expressed proteins, and unknown proteins in NCBI-GenBank.
635 EST's unique genes sequence have been identified by over 80% homology to known function in other species which were not previously described in Olea family.
Only 3.1% of total EST's was shown similarity with olive database existing in NCBI.
This generated EST's data and consensus sequences were submitted to NCBI as valuable source for functional genome studies of olive.
American Psychological Association (APA)
Ozdemir Ozgenturk, Nehir& Oruç, Fatma& Sezerman, Ugur& Kuçukural, Alper& Vural Korkut, Senay& Toksoz, Feriha…[et al.]. 2010. Generation and Analysis of Expressed Sequence Tags from Olea europaea L.. International Journal of Genomics،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-989236
Modern Language Association (MLA)
Ozdemir Ozgenturk, Nehir…[et al.]. Generation and Analysis of Expressed Sequence Tags from Olea europaea L.. International Journal of Genomics No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-989236
American Medical Association (AMA)
Ozdemir Ozgenturk, Nehir& Oruç, Fatma& Sezerman, Ugur& Kuçukural, Alper& Vural Korkut, Senay& Toksoz, Feriha…[et al.]. Generation and Analysis of Expressed Sequence Tags from Olea europaea L.. International Journal of Genomics. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-989236
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989236