Hemolysin, Protease, and EPS Producing PathogenicAeromonas hydrophila Strain An4 Shows Antibacterial Activity against Marine Bacterial Fish Pathogens
Joint Authors
Pandey, Anju
Naik, Milind
Dubey, Santosh Kumar
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Earth Science , Water and Environment
Abstract EN
A pathogenic Aeromonas hydrophila strain An4 was isolated from marine catfish and characterized with reference to its proteolytic and hemolytic activity along with SDS-PAGE profile (sodium dodecyl sulphate-Polyacrylamide gel electrophoresis) of ECPs (extracellular proteins) showing hemolysin (approximately 50 kDa).
Agar well diffusion assay using crude cell extract of the bacterial isolate clearly demonstrated antibacterial activity against indicator pathogenic bacteria, Staphylococcus arlettae strain An1, Acinetobacter sp.
strain An2, Vibrio parahaemolyticus strain An3, and Alteromonas aurentia SE3 showing inhibitory zone >10 mm well comparable to common antibiotics.
Further GC-MS analysis of crude cell extract revealed several metabolites, namely, phenolics, pyrrolo-pyrazines, pyrrolo-pyridine, and butylated hydroxytoluene (well-known antimicrobials).
Characterization of EPS using FTIR indicated presence of several protein-related amine and amide groups along with peaks corresponding to carboxylic and phenyl rings which may be attributed to its virulent and antibacterial properties, respectively.
Besides hemolysin, EPS, and protease, Aeromonas hydrophila strain An4 also produced several antibacterial metabolites.
American Psychological Association (APA)
Pandey, Anju& Naik, Milind& Dubey, Santosh Kumar. 2010. Hemolysin, Protease, and EPS Producing PathogenicAeromonas hydrophila Strain An4 Shows Antibacterial Activity against Marine Bacterial Fish Pathogens. Journal of Marine Sciences،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-989345
Modern Language Association (MLA)
Pandey, Anju…[et al.]. Hemolysin, Protease, and EPS Producing PathogenicAeromonas hydrophila Strain An4 Shows Antibacterial Activity against Marine Bacterial Fish Pathogens. Journal of Marine Sciences Vol. 2010, no. 2010 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-989345
American Medical Association (AMA)
Pandey, Anju& Naik, Milind& Dubey, Santosh Kumar. Hemolysin, Protease, and EPS Producing PathogenicAeromonas hydrophila Strain An4 Shows Antibacterial Activity against Marine Bacterial Fish Pathogens. Journal of Marine Sciences. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-989345
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989345