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Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet
Joint Authors
Engstrom, Laura W.
Bober, Loretta
Chen, Shu-Cheng
Fine, Jay S.
Li, Ying
Stanton, Michaela C.
Kinsley, David
Cui, Long
Jackson, James V.
Rojas-Triana, Alberto
Lundell, Daniel
Laverty, Maureen
Gustafson, Eric L.
Jenh, Chung-Her
Kowalski, Timothy J.
Manfra, Denise J.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-22
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The kinetics of metabolic and inflammatory parameters associated with obesity were evaluated in a murine diet-induced obesity (DIO) model using a diet high in fat and cholesterol.
Cellular infiltration and mediator production were assessed and shown to be therapeutically modulated by the PPARgamma agonist rosiglitazone.
C57BL/6 mice were maintained on a 45% fat/0.12% cholesterol (HF/CH) or Chow diet for 3, 6, 16, or 27 weeks.
Flow cytometry was employed to monitor peripheral blood monocytes and adipose tissue macrophages (ATM).
Gene expression and protein analysis methods were used to evaluate mediator production from total epididymal fat (EF), stromal vascular fraction (SVF), and sorted SVF cells.
To investigate therapeutic intervention, mice were fed a HF/CH diet for 12 weeks and then a diet formulated with rosiglitazone (5 mg/kg) for an additional 6 weeks.
A HF/CH diet correlated with obesity and a dramatic proinflammatory state.
Therapeutic intervention with rosiglitazone attenuated the HF/CH induced inflammation.
In addition, a novel population was found that expressed the highest levels of the pro-inflammatory mediators CCL2 and IL-6.
American Psychological Association (APA)
Engstrom, Laura W.& Bober, Loretta& Chen, Shu-Cheng& Fine, Jay S.& Li, Ying& Stanton, Michaela C.…[et al.]. 2010. Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet. PPAR Research،Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-989720
Modern Language Association (MLA)
Engstrom, Laura W.…[et al.]. Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet. PPAR Research No. 2010 (2010), pp.1-13.
https://search.emarefa.net/detail/BIM-989720
American Medical Association (AMA)
Engstrom, Laura W.& Bober, Loretta& Chen, Shu-Cheng& Fine, Jay S.& Li, Ying& Stanton, Michaela C.…[et al.]. Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet. PPAR Research. 2010. Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-989720
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989720