Molecular Characterization of Putative Chordoma Cell Lines
Joint Authors
Meltzer, Paul S.
Möller, Peter
Ng, David
Li, Sufeng
Osada, Takuya
Sommer, Joshua B.
Kelley, Michael J.
Brüderlein, Silke
Alcorta, David A.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-08
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Immortal tumor cell lines are an important model system for cancer research, however, misidentification and cross-contamination of cell lines are a common problem.
Seven chordoma cell lines are reported in the literature, but none has been characterized in detail.
We analyzed gene expression patterns and genomic copy number variations in five putative chordoma cell lines (U-CH1, CCL3, CCL4, GB60, and CM319).
We also created a new chordoma cell line, U-CH2, and provided genotypes for cell lines for identity confirmation.
Our analyses revealed that CCL3, CCL4, and GB60 are not chordoma cell lines, and that CM319 is a cancer cell line possibly derived from chordoma, but lacking expression of key chordoma biomarkers.
U-CH1 and U-CH2 both have gene expression profiles, copy number aberrations, and morphology consistent with chordoma tumors.
These cell lines also harbor genetic changes, such as loss of p16, MTAP, or PTEN, that make them potentially useful models for studying mechanisms of chordoma pathogenesis and for evaluating targeted therapies.
American Psychological Association (APA)
Brüderlein, Silke& Sommer, Joshua B.& Meltzer, Paul S.& Li, Sufeng& Osada, Takuya& Ng, David…[et al.]. 2010. Molecular Characterization of Putative Chordoma Cell Lines. Complexity،Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-989737
Modern Language Association (MLA)
Brüderlein, Silke…[et al.]. Molecular Characterization of Putative Chordoma Cell Lines. Complexity No. 2010 (2010), pp.1-14.
https://search.emarefa.net/detail/BIM-989737
American Medical Association (AMA)
Brüderlein, Silke& Sommer, Joshua B.& Meltzer, Paul S.& Li, Sufeng& Osada, Takuya& Ng, David…[et al.]. Molecular Characterization of Putative Chordoma Cell Lines. Complexity. 2010. Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-989737
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989737