Differential Gene Expression in Sugarcane in Response to Challenge by Fungal Pathogen Ustilago scitaminea Revealed by cDNA-AFLP
Joint Authors
Ru-Kai, Chen
You-Xiong, Que
Xian-Xian, Song
Li-Ping, Xu
Jian-Wei, Lin
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Differential gene expression in sugarcane during sugarcane-Ustilago scitaminea interaction was conducted in a smut-resistant genotype.
Using cDNA-AFLP along with silver staining, a total of 136 transcript-derived fragments (TDFs) were found to be differentially expressed in response to challenge by U.
scitaminea.
Forty TDFs, 34 newly induced plus six with obvious upregulated expression after infection, were sequenced and validated by RT-PCR analysis.
These results demonstrated that the expression of 37 out of these TDFs in RT-PCR analysis was consistent with that in cDNA-AFLP analysis.
Based on BlastX in NCBI, 28 TDFs were assumed to function in sugarcane under U.
scitaminea stress.
Analysis of expression profile of three TDFs revealed that they responded differently after infection with U.
scitaminea, and the transcription was significantly enhanced.
The response of two TDFs, SUC06 and SUC09, occurred before that of SUC10.
This study enriches our knowledge of the molecular basis for sugarcane response to U.
scitaminea infection.
American Psychological Association (APA)
You-Xiong, Que& Jian-Wei, Lin& Xian-Xian, Song& Li-Ping, Xu& Ru-Kai, Chen. 2011. Differential Gene Expression in Sugarcane in Response to Challenge by Fungal Pathogen Ustilago scitaminea Revealed by cDNA-AFLP. BioMed Research International،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-989882
Modern Language Association (MLA)
You-Xiong, Que…[et al.]. Differential Gene Expression in Sugarcane in Response to Challenge by Fungal Pathogen Ustilago scitaminea Revealed by cDNA-AFLP. BioMed Research International No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-989882
American Medical Association (AMA)
You-Xiong, Que& Jian-Wei, Lin& Xian-Xian, Song& Li-Ping, Xu& Ru-Kai, Chen. Differential Gene Expression in Sugarcane in Response to Challenge by Fungal Pathogen Ustilago scitaminea Revealed by cDNA-AFLP. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-989882
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989882