Detailed Investigations on the Solid Cell Culture and Antimicrobial Activities of the Iranian Arnebia euchroma
Joint Authors
Pourmolaei, S.
Meshkat, A.
Mousavi, A.
Akbari Noghabi, K.
Haghbeen, K.
Mareftjo, M. J.
Hosseini Shirazi, F.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In pursuit of strong shikalkin-producing cell lines, seeds of the Iranian Arnebia euchroma were collected from Dena altitudes in the central Zagross.
Chemical analysis showed that the dried root of the plant contained about 8.5% (w/w) shikalkin pigment.
The root explants of the young plantlets, obtained from the germinated seeds, were used for establishing callus.
Then, parameters effective on proliferation and pigment production of the resulting calli were studied in detail.
Accordingly, two modified media called mLS and mM9 were optimized for propagation and pigment production, respectively.
Using these media, the biomass of the A.euchroma calli was increased to 600%, and the pigment production reached to a maximum of 16.3 mg per gram of the wet biomass in a period of a subculture (21 days).
Parallel to these experiments, the antimicrobial activity of shikalkin pigment was examined on some fungi and Gram-positive and Gram-negative bacteria.
Results indicated that the pigment was almost ineffective on fungi and Gram-negative bacteria, but it was meaningfully effective against Micrococcus luteus.
American Psychological Association (APA)
Haghbeen, K.& Pourmolaei, S.& Mareftjo, M. J.& Mousavi, A.& Akbari Noghabi, K.& Hosseini Shirazi, F.…[et al.]. 2011. Detailed Investigations on the Solid Cell Culture and Antimicrobial Activities of the Iranian Arnebia euchroma. BioMed Research International،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-989884
Modern Language Association (MLA)
Haghbeen, K.…[et al.]. Detailed Investigations on the Solid Cell Culture and Antimicrobial Activities of the Iranian Arnebia euchroma. BioMed Research International No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-989884
American Medical Association (AMA)
Haghbeen, K.& Pourmolaei, S.& Mareftjo, M. J.& Mousavi, A.& Akbari Noghabi, K.& Hosseini Shirazi, F.…[et al.]. Detailed Investigations on the Solid Cell Culture and Antimicrobial Activities of the Iranian Arnebia euchroma. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-989884
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989884