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Bioactivity Determination of Native and Variant Forms of Therapeutic Interferons
Joint Authors
Diress, Abebaw
Li, Xuguang
Wang, Junzhi
Larocque, Louise
Bliu, Alex
Xu, Ranran
Lin, Rongtuan
Girard, Michel
He, Runtao
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The traditional antiviral assays for the determination of interferon potency are reported to have considerable variability between and within assays.
Although several reporter gene assays based on interferon-inducible promoter activities have been reported, data from comprehensive validation studies are lacking and few studies have been conducted to analyze the variant forms of interferons, which could have undesirable clinical implications.
Here, a reporter gene assay employing a HEK293 cell line stably transfected with luciferase gene under the control of interferon-stimulated response element promoter was developed and validated.
The assay was found to be more sensitive, with a larger detection range than the antiviral assay.
Several cytokines tested did not interfere with the test, suggesting the assay possesses a certain degree of selectivity.
Moreover, the robustness of the assay was demonstrated by minimal variations in the results generated by different analysts and cell passage number (up to 52 passages).
Finally, the method was employed to analyze several interferon variants (interferon-α 2a) and we found that the aggregated form has completely lost its potency; while a modest loss of bioactivity in oxidized interferon was observed (approx.
23%), the deamidated form essentially retained its activity.
American Psychological Association (APA)
Larocque, Louise& Bliu, Alex& Xu, Ranran& Diress, Abebaw& Wang, Junzhi& Lin, Rongtuan…[et al.]. 2011. Bioactivity Determination of Native and Variant Forms of Therapeutic Interferons. BioMed Research International،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-989890
Modern Language Association (MLA)
Larocque, Louise…[et al.]. Bioactivity Determination of Native and Variant Forms of Therapeutic Interferons. BioMed Research International No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-989890
American Medical Association (AMA)
Larocque, Louise& Bliu, Alex& Xu, Ranran& Diress, Abebaw& Wang, Junzhi& Lin, Rongtuan…[et al.]. Bioactivity Determination of Native and Variant Forms of Therapeutic Interferons. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-989890
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989890