Assessment of Elastase-Induced Murine Abdominal Aortic Aneurysms: Comparison of Ultrasound Imaging with In Situ Video Microscopy
Joint Authors
McConnell, Michael V.
Maegdefessel, Lars
Kitagawa, Toshiro
Tsao, Philip S.
Dalman, Ronald L.
Azuma, Junya
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Aims.
The aim of this study was to definitively assess the validity of noninvasive high-frequency ultrasound (US) measurements of aortic luminal diameter (ALD) in a murine model of elastase-induced abdominal aortic aneurysm in comparison with in situ video microscopy (VM).
Methods.
C57BL/6 mice underwent transient perfusion of the aorta with either elastase (n=20: Elastase group) or saline (n=10: Sham).
Unoperated mice (n=10) were also studied.
Results.
ALD measurements by US had excellent linear correlation and absolute agreement with that by VM in both Control (unoperated or sham-operated mice) and elastase groups (r=0.96, intraclass correlation coefficient (ICC)=0.88 and r=0.93, ICC=0.92, resp.).
Bland-Altman analysis of US compared with VM measurements in both groups indicated good agreement, however US measurements were slightly but significantly higher than VM measurements in the control group (mean bias 0.039 mm, P<.05).
Linear regression analysis revealed excellent correlation between US and VM measurements in both groups.
(R2=0.91 in Control group, R2=0.85 in elastase group.) The reliability of US measurements was also confirmed by ex vivo histological measurements.
Conclusions.
High-frequency US provides reliable ALD measurements in developing murine abdominal aortic aneurysms.
American Psychological Association (APA)
Azuma, Junya& Maegdefessel, Lars& Kitagawa, Toshiro& Dalman, Ronald L.& McConnell, Michael V.& Tsao, Philip S.. 2010. Assessment of Elastase-Induced Murine Abdominal Aortic Aneurysms: Comparison of Ultrasound Imaging with In Situ Video Microscopy. BioMed Research International،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-989954
Modern Language Association (MLA)
Azuma, Junya…[et al.]. Assessment of Elastase-Induced Murine Abdominal Aortic Aneurysms: Comparison of Ultrasound Imaging with In Situ Video Microscopy. BioMed Research International No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-989954
American Medical Association (AMA)
Azuma, Junya& Maegdefessel, Lars& Kitagawa, Toshiro& Dalman, Ronald L.& McConnell, Michael V.& Tsao, Philip S.. Assessment of Elastase-Induced Murine Abdominal Aortic Aneurysms: Comparison of Ultrasound Imaging with In Situ Video Microscopy. BioMed Research International. 2010. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-989954
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989954