New Method to Disaggregate and Analyze Single Isolated Helminthes Cells Using Flow Cytometry: Proof of Concept
Joint Authors
Morales-Montor, Jorge
Hernández-Bello, Romel
Nava-Castro, Karen
Escobedo, Galileo
Muñiz-Hernández, Saé
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In parasitology, particularly in helminthes studies, several methods have been used to look for the expression of specific molecules, such as RT-PCR, western blot, 2D-electrophoresis, and microscopy, among others.
However, these methods require homogenization of the whole helminth parasite, preventing evaluation of individual cells or specific cell types in a given parasite tissue or organ.
Also, the extremely high interaction between helminthes and host cells (particularly immune cells) is an important point to be considered.
It is really hard to obtain fresh parasites without host cell contamination.
Then, it becomes crucial to determine that the analyzed proteins are exclusively from parasitic origin, and not a consequence of host cell contamination.
Flow cytometry is a fluorescence-based technique used to evaluate the expression of extra-and intracellular proteins in different type cells, including protozoan parasites.
It also allows the isolation and recovery of single-cell populations.
Here, we describe a method to isolate and obtain purified helminthes cells.
American Psychological Association (APA)
Nava-Castro, Karen& Hernández-Bello, Romel& Muñiz-Hernández, Saé& Escobedo, Galileo& Morales-Montor, Jorge. 2011. New Method to Disaggregate and Analyze Single Isolated Helminthes Cells Using Flow Cytometry: Proof of Concept. BioMed Research International،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-989956
Modern Language Association (MLA)
Nava-Castro, Karen…[et al.]. New Method to Disaggregate and Analyze Single Isolated Helminthes Cells Using Flow Cytometry: Proof of Concept. BioMed Research International No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-989956
American Medical Association (AMA)
Nava-Castro, Karen& Hernández-Bello, Romel& Muñiz-Hernández, Saé& Escobedo, Galileo& Morales-Montor, Jorge. New Method to Disaggregate and Analyze Single Isolated Helminthes Cells Using Flow Cytometry: Proof of Concept. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-989956
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989956