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Herpes Simplex Virus Type-1 (HSV-1) Entry into Human Mesenchymal Stem Cells Is Heavily Dependent on Heparan Sulfate
Joint Authors
Alencastro, Frances
Choudhary, Samiksha
Tiwari, Vaibhav
Zhao, Yuanxiang
Marquez, Maribel
Spors, Frank
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-14
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Hematopoietic stem cells recipients remain susceptible to opportunistic viral infections including herpes simplex virus type-1 (HSV-1).
The purpose of this investigation was to analyze susceptibility of human mesenchymal stem cells (hMSCs) to HSV-1 infection and identify the major entry receptor.
Productive virus infection in hMSCs was confirmed by replication and plaque formation assays using a syncytial HSV-1 KOS (804) virus.
To examine the significance of entry receptors, RT-PCR and antibody-blocking assays were performed.
RT-PCR data showed the expression of gD receptors: nectin-1, 3-O sulfotransferase-3 (3-OST-3), and HVEM.
Antibody-blocking assay together with heparinase treatment suggested an important role for HS and 3-O-sulfated heparan sulfate (3-OS HS), but not nectin-1 or HVEM, in mediating HSV-1 entry and spread in hMSCs.
Taken together, our results provide strong evidence demonstrating that HSV-1 is capable of infecting hMSCs and HS and 3-OS HS serve as its entry receptors during this process.
American Psychological Association (APA)
Choudhary, Samiksha& Marquez, Maribel& Alencastro, Frances& Spors, Frank& Zhao, Yuanxiang& Tiwari, Vaibhav. 2011. Herpes Simplex Virus Type-1 (HSV-1) Entry into Human Mesenchymal Stem Cells Is Heavily Dependent on Heparan Sulfate. BioMed Research International،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-989959
Modern Language Association (MLA)
Choudhary, Samiksha…[et al.]. Herpes Simplex Virus Type-1 (HSV-1) Entry into Human Mesenchymal Stem Cells Is Heavily Dependent on Heparan Sulfate. BioMed Research International No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-989959
American Medical Association (AMA)
Choudhary, Samiksha& Marquez, Maribel& Alencastro, Frances& Spors, Frank& Zhao, Yuanxiang& Tiwari, Vaibhav. Herpes Simplex Virus Type-1 (HSV-1) Entry into Human Mesenchymal Stem Cells Is Heavily Dependent on Heparan Sulfate. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-989959
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989959