Identification of NF-κB Modulation Capabilities within Human Intestinal Commensal Bacteria
Joint Authors
Tap, Julien
Blottière, Hervé M.
Doré, Joël
Lakhdari, Omar
de Wouters, Tomas
Le Roux, Karine
Béguet-Crespel, Fabienne
Cultrone, Antonietta
Lefèvre, Fabrice
Nepelska, Malgorzata
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The intestinal microbiota plays an important role in modulation of mucosal immune responses.
To seek interactions between intestinal epithelial cells (IEC) and commensal bacteria, we screened 49 commensal strains for their capacity to modulate NF-κB.
We used HT-29/kb-seap-25 and Caco-2/kb-seap-7 intestinal epithelial cells and monocyte-like THP-1 blue reporter cells to measure effects of commensal bacteria on cellular expression of a reporter system for NF-κB.
Bacteria conditioned media (CM) were tested alone or together with an activator of NF-κB to explore its inhibitory potentials.
CM from 8 or 10 different commensal species activated NF-κB expression on HT-29 and Caco-2 cells, respectively.
On THP-1, CM from all but 5 commensal strains stimulated NF-κB.
Upon challenge with TNF-α or IL-1β, some CM prevented induced NF-κB activation, whereas others enhanced it.
Interestingly, the enhancing effect of some CM was correlated with the presence of butyrate and propionate.
Characterization of the effects of the identified bacteria and their implications in human health awaits further investigations.
American Psychological Association (APA)
Lakhdari, Omar& Tap, Julien& Béguet-Crespel, Fabienne& Le Roux, Karine& de Wouters, Tomas& Cultrone, Antonietta…[et al.]. 2011. Identification of NF-κB Modulation Capabilities within Human Intestinal Commensal Bacteria. BioMed Research International،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-989973
Modern Language Association (MLA)
Lakhdari, Omar…[et al.]. Identification of NF-κB Modulation Capabilities within Human Intestinal Commensal Bacteria. BioMed Research International No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-989973
American Medical Association (AMA)
Lakhdari, Omar& Tap, Julien& Béguet-Crespel, Fabienne& Le Roux, Karine& de Wouters, Tomas& Cultrone, Antonietta…[et al.]. Identification of NF-κB Modulation Capabilities within Human Intestinal Commensal Bacteria. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-989973
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989973