BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes
Joint Authors
Šafář, Jan
Suchánková, Pavla
Šimková, Hana
Doležel, Jaroslav
Bartoš, Jan
Luo, Ming-Cheng
Peng, Junhua
Ma, Yaqin
Weng, Yiqun
You, Frank M.
Kubaláková, Marie
Lapitan, Nora L.V.
Číhalíková, Jarmila
Robert-Quatre, Heda
Azhaguvel, Perumal
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Positional cloning in bread wheat is a tedious task due to its huge genome size and hexaploid character.
BAC libraries represent an essential tool for positional cloning.
However, wheat BAC libraries comprise more than million clones, which makes their screening very laborious.
Here, we present a targeted approach based on chromosome-specific BAC libraries.
Such libraries were constructed from flow-sorted arms of wheat chromosome 7D.
A library from the short arm (7DS) consisting of 49,152 clones with 113 kb insert size represented 12.1 arm equivalents whereas a library from the long arm (7DL) comprised 50,304 clones of 116 kb providing 14.9x arm coverage.
The 7DS library was PCR screened with markers linked to Russian wheat aphid resistance gene DnCI2401, the 7DL library was screened by hybridization with a probe linked to greenbug resistance gene Gb3.
The small number of clones combined with high coverage made the screening highly efficient and cost effective.
American Psychological Association (APA)
Šimková, Hana& Šafář, Jan& Kubaláková, Marie& Suchánková, Pavla& Číhalíková, Jarmila& Robert-Quatre, Heda…[et al.]. 2010. BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes. BioMed Research International،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-989996
Modern Language Association (MLA)
Šimková, Hana…[et al.]. BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes. BioMed Research International No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-989996
American Medical Association (AMA)
Šimková, Hana& Šafář, Jan& Kubaláková, Marie& Suchánková, Pavla& Číhalíková, Jarmila& Robert-Quatre, Heda…[et al.]. BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes. BioMed Research International. 2010. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-989996
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989996