Possible Linkage of SP6 Transcriptional Activity with Amelogenesis by Protein Stabilization
Joint Authors
Hagita, Hiroko
Yanuaryska, Ryna Dwi
Utami, Trianna W.
Horiguchi, Taigo
Miyoshi, Keiko
Noma, Takafumi
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Ameloblasts produce enamel matrix proteins such as amelogenin, ameloblastin, and amelotin during tooth development.
The molecular mechanisms of ameloblast differentiation (amelogenesis) are currently not well understood.
SP6 is a transcription factor of the Sp/KLF family that was recently found to regulate cell proliferation in a cell-type-specific manner.
Sp6-deficient mice demonstrate characteristic tooth anomalies such as delayed eruption of the incisors and supernumerary teeth with disorganized amelogenesis.
However, it remains unclear how Sp6 controls amelogenesis.
In this study, we used SP6 high producer cells to identify SP6 target genes.
Based on the observations that long-term culture of SP6 high producer cells reduced SP6 protein expression but not Sp6 mRNA expression, we found that SP6 is short lived and specifically degraded through a proteasome pathway.
We established an in vitro inducible SP6 expression system coupled with siRNA knockdown and found a possible linkage between SP6 and amelogenesis through the regulation of amelotin and Rock1 gene expression by microarray analysis.
Our findings suggest that the regulation of SP6 protein stability is one of the crucial steps in amelogenesis.
American Psychological Association (APA)
Utami, Trianna W.& Miyoshi, Keiko& Hagita, Hiroko& Yanuaryska, Ryna Dwi& Horiguchi, Taigo& Noma, Takafumi. 2011. Possible Linkage of SP6 Transcriptional Activity with Amelogenesis by Protein Stabilization. BioMed Research International،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-990009
Modern Language Association (MLA)
Utami, Trianna W.…[et al.]. Possible Linkage of SP6 Transcriptional Activity with Amelogenesis by Protein Stabilization. BioMed Research International No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-990009
American Medical Association (AMA)
Utami, Trianna W.& Miyoshi, Keiko& Hagita, Hiroko& Yanuaryska, Ryna Dwi& Horiguchi, Taigo& Noma, Takafumi. Possible Linkage of SP6 Transcriptional Activity with Amelogenesis by Protein Stabilization. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-990009
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990009