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Using Bacterial Artificial Chromosomes in Leukemia Research: The Experience at the University Cytogenetics Laboratory in Brest,France
Joint Authors
Férec, Claude
Basinko, Audrey
De Braekeleer, Marc
Morel, Frédéric
De Braekeleer, Etienne
Douet-Guilbert, Nathalie
Le Bris, Marie-Josée
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The development of the bacterial artificial chromosome (BAC) system was driven in part by the human genome project in order to construct genomic DNA libraries and physical maps for genomic sequencing.
The availability of BAC clones has become a valuable tool for identifying cancer genes.
We report here our experience in identifying genes located at breakpoints of chromosomal rearrangements and in defining the size and boundaries of deletions in hematological diseases.
The methodology used in our laboratory consists of a three-step approach using conventional cytogenetics followed by FISH with commercial probes, then BAC clones.
One limitation to the BAC system is that it can only accommodate inserts of up to 300 kb.
As a consequence, analyzing the extent of deletions requires a large amount of material.
Array comparative genomic hybridization (array-CGH) using a BAC/PAC system can be an alternative.
However, this technique has limitations also, and it cannot be used to identify candidate genes at breakpoints of chromosomal rearrangements such as translocations, insertions, and inversions.
American Psychological Association (APA)
De Braekeleer, Etienne& Douet-Guilbert, Nathalie& Basinko, Audrey& Morel, Frédéric& Le Bris, Marie-Josée& Férec, Claude…[et al.]. 2010. Using Bacterial Artificial Chromosomes in Leukemia Research: The Experience at the University Cytogenetics Laboratory in Brest,France. BioMed Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990011
Modern Language Association (MLA)
De Braekeleer, Etienne…[et al.]. Using Bacterial Artificial Chromosomes in Leukemia Research: The Experience at the University Cytogenetics Laboratory in Brest,France. BioMed Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-990011
American Medical Association (AMA)
De Braekeleer, Etienne& Douet-Guilbert, Nathalie& Basinko, Audrey& Morel, Frédéric& Le Bris, Marie-Josée& Férec, Claude…[et al.]. Using Bacterial Artificial Chromosomes in Leukemia Research: The Experience at the University Cytogenetics Laboratory in Brest,France. BioMed Research International. 2010. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990011
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990011