Ovarian Surface Epithelium in Patients with Severe Ovarian Infertility: A Potential Source of Cells Expressing Markers of PluripotentMultipotent Stem Cells
Joint Authors
Skutella, Thomas
Stimpfel, Martin
Virant-Klun, Irma
Sinkovec, Jasna
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-06
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The aim of this study was to confirm the presence of stem cells in the ovarian surface epithelium of patients with premature ovarian failure and no mature follicles and oocytes.
In these patients, small round cells of unknown origin expressing SOX-2 marker of pluripotency were observed among the epithelial cells just after the ovarian surface epithelium scraping.
These cells were an integral part of the ovarian surface epithelium.
When the scraped cells were cultured in a medium with added follicular fluid to provide some ovarian niche, primitive oocyte-like cells and typical round-shaped cell clusters positively stained on alkaline phosphatase, and markers of pluripotency, such as SOX-2 and SSEA-4, were developed.
These markers were expressed early and also later in the culture.
Single oocyte-like cells expressed genes OCT4A, SOX-2, NANOG, NANOS, STELLA, CD9, LIN28, KLF4, GDF3, and MYC, characteristic for pluripotent stem cells.
The results of this study confirmed the presence of putative stem cells in the ovarian surface epithelium of these patients and provided some basis to create a stem cell line in the future.
American Psychological Association (APA)
Virant-Klun, Irma& Skutella, Thomas& Stimpfel, Martin& Sinkovec, Jasna. 2011. Ovarian Surface Epithelium in Patients with Severe Ovarian Infertility: A Potential Source of Cells Expressing Markers of PluripotentMultipotent Stem Cells. BioMed Research International،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-990049
Modern Language Association (MLA)
Virant-Klun, Irma…[et al.]. Ovarian Surface Epithelium in Patients with Severe Ovarian Infertility: A Potential Source of Cells Expressing Markers of PluripotentMultipotent Stem Cells. BioMed Research International No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-990049
American Medical Association (AMA)
Virant-Klun, Irma& Skutella, Thomas& Stimpfel, Martin& Sinkovec, Jasna. Ovarian Surface Epithelium in Patients with Severe Ovarian Infertility: A Potential Source of Cells Expressing Markers of PluripotentMultipotent Stem Cells. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-990049
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990049