The Selection and Use of Sorghum (Sorghum propinquum) Bacterial Artificial Chromosomes as Cytogenetic FISH Probes for Maize (Zea mays L.)
Joint Authors
Beckham, Katherine D.
Strobel, Cornelia
Davis, James D.
Ring, Brian C.
Bass, Hank W.
Conejo, Maria S.
Figueroa, Debbie M.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-08
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
The integration of genetic and physical maps of maize is progressing rapidly, but the cytogenetic maps lag behind, with the exception of the pachytene fluorescence in situ hybridization (FISH) maps of maize chromosome 9.
We sought to produce integrated FISH maps of other maize chromosomes using Core Bin Marker loci.
Because these 1 Kb restriction fragment length polymorphism (RFLP) probes are below the FISH detection limit, we used BACs from sorghum, a small-genome relative of maize, as surrogate clones for FISH mapping.
We sequenced 151 maize RFLP probes and compared in silico BAC selection methods to that of library filter hybridization and found the latter to be the best.
BAC library screening, clone verification, and single-clone selection criteria are presented along with an example of transgenomic BAC FISH mapping.
This strategy has been used to facilitate the integration of RFLP and FISH maps in other large-genome species.
American Psychological Association (APA)
Figueroa, Debbie M.& Davis, James D.& Strobel, Cornelia& Conejo, Maria S.& Beckham, Katherine D.& Ring, Brian C.…[et al.]. 2010. The Selection and Use of Sorghum (Sorghum propinquum) Bacterial Artificial Chromosomes as Cytogenetic FISH Probes for Maize (Zea mays L.). BioMed Research International،Vol. 2011, no. 2011, pp.1-16.
https://search.emarefa.net/detail/BIM-990056
Modern Language Association (MLA)
Figueroa, Debbie M.…[et al.]. The Selection and Use of Sorghum (Sorghum propinquum) Bacterial Artificial Chromosomes as Cytogenetic FISH Probes for Maize (Zea mays L.). BioMed Research International No. 2011 (2011), pp.1-16.
https://search.emarefa.net/detail/BIM-990056
American Medical Association (AMA)
Figueroa, Debbie M.& Davis, James D.& Strobel, Cornelia& Conejo, Maria S.& Beckham, Katherine D.& Ring, Brian C.…[et al.]. The Selection and Use of Sorghum (Sorghum propinquum) Bacterial Artificial Chromosomes as Cytogenetic FISH Probes for Maize (Zea mays L.). BioMed Research International. 2010. Vol. 2011, no. 2011, pp.1-16.
https://search.emarefa.net/detail/BIM-990056
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990056