Adherent Primary Cultures of Mouse Intercostal Muscle Fibers for Isolated Fiber Studies
Joint Authors
Schneider, Martin F.
Robison, Patrick
Hernández-Ochoa, Erick O.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Primary culture models of single adult skeletal muscle fibers dissociated from locomotor muscles adhered to glass coverslips are routine and allow monitoring of functional processes in living cultured fibers.
To date, such isolated fiber cultures have not been established for respiratory muscles, despite the fact that dysfunction of core respiratory muscles leading to respiratory arrest is the most common cause of death in many muscular diseases.
Here we present the first description of an adherent culture system for single adult intercostal muscle fibers from the adult mouse.
This system allows for monitoring functional properties of these living muscle fibers in culture with or without electrical field stimulation to drive muscle fiber contraction at physiological or pathological respiratory firing patterns.
We also provide initial characterization of these fibers, demonstrating several common techniques in this new model system in the context of the established Flexor Digitorum Brevis muscle primary culture model.
American Psychological Association (APA)
Robison, Patrick& Hernández-Ochoa, Erick O.& Schneider, Martin F.. 2011. Adherent Primary Cultures of Mouse Intercostal Muscle Fibers for Isolated Fiber Studies. BioMed Research International،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-990059
Modern Language Association (MLA)
Robison, Patrick…[et al.]. Adherent Primary Cultures of Mouse Intercostal Muscle Fibers for Isolated Fiber Studies. BioMed Research International No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-990059
American Medical Association (AMA)
Robison, Patrick& Hernández-Ochoa, Erick O.& Schneider, Martin F.. Adherent Primary Cultures of Mouse Intercostal Muscle Fibers for Isolated Fiber Studies. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-990059
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990059