Pathogenicity of a Very Virulent Strain of Marek's Disease Herpesvirus Cloned as Infectious Bacterial Artificial Chromosomes
Joint Authors
Smith, Lorraine P.
Petherbridge, Lawrence J.
Nair, Venugopal
Baigent, Susan J.
Simpson, Jennifer
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Bacterial artificial chromosome (BAC) vectors containing the full-length genomes of several herpesviruses have been used widely as tools to enable functional studies of viral genes.
Marek's disease viruses (MDVs) are highly oncogenic alphaherpesviruses that induce rapid-onset T-cell lymphomas in chickens.
Oncogenic strains of MDV reconstituted from BAC clones have been used to examine the role of viral genes in inducing tumours.
Past studies have demonstrated continuous increase in virulence of MDV strains.
We have previously reported on the UK isolate C12/130 that showed increased virulence features including lymphoid organ atrophy and enhanced tropism for the central nervous system.
Here we report the construction of the BAC clones (pC12/130) of this strain.
Chickens were infected with viruses reconstituted from the pC12/130 clones along with the wild-type virus for the comparison of the pathogenic properties.
Our studies show that BAC-derived viruses induced disease similar to the wild-type virus, though there were differences in the levels of pathogenicity between individual viruses.
Generation of BAC clones that differ in the potential to induce cytolytic disease provide the opportunity to identify the molecular determinants of increased virulence by direct sequence analysis as well as by using reverse genetics approaches on the infectious BAC clones.
American Psychological Association (APA)
Smith, Lorraine P.& Petherbridge, Lawrence J.& Baigent, Susan J.& Simpson, Jennifer& Nair, Venugopal. 2010. Pathogenicity of a Very Virulent Strain of Marek's Disease Herpesvirus Cloned as Infectious Bacterial Artificial Chromosomes. BioMed Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990081
Modern Language Association (MLA)
Smith, Lorraine P.…[et al.]. Pathogenicity of a Very Virulent Strain of Marek's Disease Herpesvirus Cloned as Infectious Bacterial Artificial Chromosomes. BioMed Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-990081
American Medical Association (AMA)
Smith, Lorraine P.& Petherbridge, Lawrence J.& Baigent, Susan J.& Simpson, Jennifer& Nair, Venugopal. Pathogenicity of a Very Virulent Strain of Marek's Disease Herpesvirus Cloned as Infectious Bacterial Artificial Chromosomes. BioMed Research International. 2010. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990081
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990081