Construction and Characterization of a Bacterial Artificial Chromosome Library for the A-Genome of Cotton (G. arboreum L.)‎

Abstract EN

A bacterial artificial chromosome (BAC) library for the A-genome of cotton has been constructed from the leaves of G.

arboreum L cv.

Jianglinzhongmian.

It is used as elite A-genome germplasm resources in the present cotton breeding program and has been used to build a genetic reference map of cotton.

The BAC library consists of 123,648 clones stored in 322 384-well plates.

Statistical analysis of a set of 103 randomly selected BAC clones indicated that each clone has an average insert length of 100.2 kb per plasmid, with a range of 30 to 190 kb.

Theoretically, this represents 7.2 haploid genome equivalents based on an A-genome size of 1697 Mb.

The BAC library has been arranged in column pools and superpools allowing screening with various PCR-based markers.

In the future, the A-genome cotton BAC library will serve as both a giant gene resource and a valuable tool for map-based gene isolation, physical mapping and comparative genome analysis.