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Higher Molecular Weight Polyethylene Glycol Increases Cell Proliferation While Improving Barrier Function in an In Vitro Colon Cancer Model
Joint Authors
Glover, Sarah C.
Vishnubhotla, Ramana
Sun, Shan
Cho, Michael
Bharadwaj, Shruthi
Chauhan, Chinmay
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Polyethylene glycol (PEG) has been previously shown to protect against enteric pathogens and prevent colon cancer invasion.
To determine if PEG could indeed protect against previously observed pro-invasive effects of commensal E.
coli and EPEC, Caco-2 cells grown in an in vitro model of colon cancer were infected with strains of human commensal E.
coli or EPEC and treated with 10% PEG 3350, PEG 8000, and PEG 20,000, respectively.
At 24 hours after infection, MMP-1 and MMP-13 activities, cell cluster thickness, depth of invasion, and proliferation were determined using standard molecular biology techniques and advanced imaging.
We found that higher molecular weight PEG, especially PEG 8000 and 20,000, regardless of bacterial infection, increased proliferation and depth of invasion although a decrease in cellular density and MMP-1 activity was also noted.
Maximum proliferation and depth of invasion of Caco-2 cells was observed in scaffolds treated with a combination of commensal E.
coli strain, HS4 and PEG 8000.
In conclusion, we found that PEG 8000 increased cell proliferation and led to the preservation of cell density in cells treated with commensal bacteria.
This is important, because the preservation of a proliferative response in colon cancer results in a more chemo-responsive tumor.
American Psychological Association (APA)
Bharadwaj, Shruthi& Vishnubhotla, Ramana& Sun, Shan& Chauhan, Chinmay& Cho, Michael& Glover, Sarah C.. 2011. Higher Molecular Weight Polyethylene Glycol Increases Cell Proliferation While Improving Barrier Function in an In Vitro Colon Cancer Model. BioMed Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990208
Modern Language Association (MLA)
Bharadwaj, Shruthi…[et al.]. Higher Molecular Weight Polyethylene Glycol Increases Cell Proliferation While Improving Barrier Function in an In Vitro Colon Cancer Model. BioMed Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-990208
American Medical Association (AMA)
Bharadwaj, Shruthi& Vishnubhotla, Ramana& Sun, Shan& Chauhan, Chinmay& Cho, Michael& Glover, Sarah C.. Higher Molecular Weight Polyethylene Glycol Increases Cell Proliferation While Improving Barrier Function in an In Vitro Colon Cancer Model. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990208
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990208