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Does Valproic Acid Induce Neuroendocrine Differentiation in Prostate Cancer?
Joint Authors
Carducci, Michael A.
Toubaji, Antoun
Rodriguez, Ronald
Wang, Muwen
Lupold, Shawn E.
Shabbeer, Shabana
Chowdhury, Wasim H.
Netto, George
Sidana, Abhinav
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Valproic Acid (VPA) is a histone deacetylase inhibitor that holds promise for cancer therapy.
Here, we investigate whether VPA treatment induces neuroendocrine differentiation of Prostate Cancer (PCa).
A tissue microarray of VPA-treated and untreated tumor xenografts and cell lines of human PCa (LNCaP, C4-2, DU145, and PC-3) were generated and were analyzed by immunohistochemical analysis (IHC) for NE markers chromogranin A (CgA), synaptophysin, and NCAM (neural cell adhesion molecule).
Western blot analysis for CgA was performed to confirm the results of the TMA.
IHC analysis did not reveal any induction of CgA, synaptophysin, or NCAM in any xenograft after VPA treatment in vivo.
In vitro, VPA treatment induced little synaptophysin expression in C4-2 and PC-3 cells and NCAM expression in LNCaP and PC-3 cells.
In the case of CgA, VPA treatment decreased its expression in vitro in a dose-dependent manner, as determined by western blot analysis.
Thus our data demonstrates that VPA does not induce NE differentiation of PCa cells in the physiologically relevant in vivo setting.
American Psychological Association (APA)
Sidana, Abhinav& Wang, Muwen& Chowdhury, Wasim H.& Toubaji, Antoun& Shabbeer, Shabana& Netto, George…[et al.]. 2010. Does Valproic Acid Induce Neuroendocrine Differentiation in Prostate Cancer?. BioMed Research International،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-990216
Modern Language Association (MLA)
Sidana, Abhinav…[et al.]. Does Valproic Acid Induce Neuroendocrine Differentiation in Prostate Cancer?. BioMed Research International No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-990216
American Medical Association (AMA)
Sidana, Abhinav& Wang, Muwen& Chowdhury, Wasim H.& Toubaji, Antoun& Shabbeer, Shabana& Netto, George…[et al.]. Does Valproic Acid Induce Neuroendocrine Differentiation in Prostate Cancer?. BioMed Research International. 2010. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-990216
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990216