MYC, TP53, and Chromosome 17 Copy-Number Alterations in Multiple Gastric Cancer Cell Lines and in Their Parental Primary Tumors
Joint Authors
Calcagno, Danielle Queiroz
Burbano, Rommel Rodríguez
de Arruda Cardoso Smith, Marília
Silva, Tanielly Cristina Raiol
Khayat, André Salim
Leal, Mariana Ferreira
Assumpção, Paulo Pimentel
Chen, Elizabeth Suchi
Borges da Costa, Joana de Fátima Ferreira
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-01-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We evaluated whether MYC, TP53, and chromosome 17 copy-number alterations occur in ACP02, ACP03, and AGP01 gastric cancer cell lines and in their tumor counterpart.
Fluorescence in situ hybridization for MYC and TP53 genes and for chromosome 17 was applied in the 6th, 12th, 60th, and 85th passages of the cell lines and in their parental primary tumors.
We observed that three and four MYC signals were the most common alterations in gastric cell lines and tumors.
ACP02 presented cells with two copies of chr17 and loss of one copy of TP53 more frequently than ACP03 and AGP01.
Only ACP03 and AGP01 presented clonal chr17 trisomy with three or two TP53 copies.
The frequency of MYC gain, TP53 loss, and chromosome 17 trisomy seems to increase in gastric cell lines compared to their parental tumors.
Our findings reveal that these cell lines retain, in vitro, the genetic alterations presented in their parental primary tumors.
American Psychological Association (APA)
Leal, Mariana Ferreira& Calcagno, Danielle Queiroz& Borges da Costa, Joana de Fátima Ferreira& Silva, Tanielly Cristina Raiol& Khayat, André Salim& Chen, Elizabeth Suchi…[et al.]. 2011. MYC, TP53, and Chromosome 17 Copy-Number Alterations in Multiple Gastric Cancer Cell Lines and in Their Parental Primary Tumors. BioMed Research International،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990222
Modern Language Association (MLA)
Leal, Mariana Ferreira…[et al.]. MYC, TP53, and Chromosome 17 Copy-Number Alterations in Multiple Gastric Cancer Cell Lines and in Their Parental Primary Tumors. BioMed Research International No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-990222
American Medical Association (AMA)
Leal, Mariana Ferreira& Calcagno, Danielle Queiroz& Borges da Costa, Joana de Fátima Ferreira& Silva, Tanielly Cristina Raiol& Khayat, André Salim& Chen, Elizabeth Suchi…[et al.]. MYC, TP53, and Chromosome 17 Copy-Number Alterations in Multiple Gastric Cancer Cell Lines and in Their Parental Primary Tumors. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990222
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990222