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Manipulation of pH Shift to Enhance the Growth and Antibiotic Activity of Xenorhabdus nematophila
Joint Authors
Zhang, Xing
Cheng, Yongpeng
Fang, Xiangling
Wang, Yonghong
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
To evaluate the effects of pH control strategy on cell growth and the production of antibiotic (cyclo(2-Me-BABA-Gly)) by Xenorhabdus nematophila and enhance the antibiotic activity.
The effects of uncontrolled- (different initial pH) and controlled-pH (different constant pH and pH-shift) operations on cell growth and antibiotic activity of X.
nematophila YL00I were examined.
Experiments showed that the optimal initial pH for cell growth and antibiotic production of X.
nematophila YL001 occurred at 7.0.
Under different constant pH, a pH level of 7.5 was found to be optimal for biomass and antibiotic activity at 23.71 g/L and 100.0 U/mL, respectively.
Based on the kinetic information relating to the different constant pH effects on the fermentation of X.
nematophila YL001, a two-stage pH control strategy in which pH 6.5 was maintained for the first 24 h, and then switched to 7.5 after 24 h, was established to improve biomass production and antibiotic activity.
By applying this pH-shift strategy, the maximal antibiotic activity and productivity were significantly improved and reaching 185.0 U/mL and 4.41 U/mL/h, respectively, compared to values obtained from constant pH operation (100.0 U/mL and 1.39 U/mL/h).
American Psychological Association (APA)
Wang, Yonghong& Fang, Xiangling& Cheng, Yongpeng& Zhang, Xing. 2011. Manipulation of pH Shift to Enhance the Growth and Antibiotic Activity of Xenorhabdus nematophila. BioMed Research International،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-990237
Modern Language Association (MLA)
Wang, Yonghong…[et al.]. Manipulation of pH Shift to Enhance the Growth and Antibiotic Activity of Xenorhabdus nematophila. BioMed Research International No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-990237
American Medical Association (AMA)
Wang, Yonghong& Fang, Xiangling& Cheng, Yongpeng& Zhang, Xing. Manipulation of pH Shift to Enhance the Growth and Antibiotic Activity of Xenorhabdus nematophila. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-990237
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990237