Employment of Oligodeoxynucleotide plus Interleukin-2 Improves Cytogenetic Analysis in Splenic Marginal Zone Lymphoma
Joint Authors
Musto, Pellegrino
Abass, Awad E.
Tammiso, Elisa
Volta, Eleonora
Rigolin, Gian Matteo
Cuneo, Antonio
Pezzolo, Elisa
Cavazzini, Francesco
Rizzotto, Lara
D'Auria, Fiorella
Daghia, Giulia
Sofritti, Olga
Formigaro, Luca
Ambrosio, Cristina
Bardi, Antonella
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
To compare the efficiency of novel mitogenic agents and traditional mitosis inductors, 18 patients with splenic marginal zone lymphoma (SMZL) were studied.
Three cultures using oligodeoxynucleotide (ODN) plus interleukin-2 (IL-2), or TPA, or LPS were setup in each patient.
Seventeen/18 cases with ODN+IL2 had moderate/good proliferation (94,4%) as compared with 10/18 cases with TPA and LPS (55%) (P=.015); 14/18 (77,7%) cases with ODN+IL2 had sufficient good quality of banding as compared with 8/18 cases (44,4%) with TPA and LPS.
The karyotype could be defined from ODN+IL2-stimulated cultures in all 18 patients, 14 of whom (77,7%) had a cytogenetic aberration, whereas clonal aberrations could be documented in 9 and in 3 cases by stimulation with LPS and TPA, respectively.
Recurrent chromosome aberrations in our series were represented by aberrations of chromosome 14q in 5 patients, by trisomy 12 and 7q deletion in 4 cases each, and by abnormalities involving 11q and 13q in two cases each.
These findings show that stimulation with ODN+IL2 offers more mitotic figures of better quality and results in an increased rate of clonal aberrations in SMZL, making this method ideal for prospective studies aiming at the definition of the prognostic impact of cytogenetic aberrations in this disorder.
American Psychological Association (APA)
Bardi, Antonella& Cavazzini, Francesco& Rigolin, Gian Matteo& Tammiso, Elisa& Volta, Eleonora& Pezzolo, Elisa…[et al.]. 2011. Employment of Oligodeoxynucleotide plus Interleukin-2 Improves Cytogenetic Analysis in Splenic Marginal Zone Lymphoma. BioMed Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990248
Modern Language Association (MLA)
Bardi, Antonella…[et al.]. Employment of Oligodeoxynucleotide plus Interleukin-2 Improves Cytogenetic Analysis in Splenic Marginal Zone Lymphoma. BioMed Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-990248
American Medical Association (AMA)
Bardi, Antonella& Cavazzini, Francesco& Rigolin, Gian Matteo& Tammiso, Elisa& Volta, Eleonora& Pezzolo, Elisa…[et al.]. Employment of Oligodeoxynucleotide plus Interleukin-2 Improves Cytogenetic Analysis in Splenic Marginal Zone Lymphoma. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990248
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990248