Chromosomal Rearrangements in Post-Chernobyl Papillary Thyroid Carcinomas: Evaluation by Spectral Karyotyping and Automated Interphase FISH
Joint Authors
Thomas, Geraldine
Bogdanova, Tatjana
Zitzelsberger, Horst
Hieber, Ludwig
Braselmann, Herbert
Bauer, Verena
Huber, Reinhard
Schäffner, Quirin
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Structural genomic rearrangements are frequent findings in human cancers.
Therefore, papillary thyroid carcinomas (PTCs) were investigated for chromosomal aberrations and rearrangements of the RET proto-oncogene.
For this purpose, primary cultures from 23 PTC have been established and metaphase preparations were analysed by spectral karyotyping (SKY).
In addition, interphase cell preparations of the same cases were investigated by fluorescence in situ hybridisation (FISH) for the presence of RET/PTC rearrangements using RET-specific DNA probes.
SKY analysis of PTC revealed structural aberrations of chromosome 11 and several numerical aberrations with frequent loss of chromosomes 20, 21, and 22.
FISH analysis for RET/PTC rearrangements showed prevalence of this rearrangement in 72% (16 out of 22) of cases.
However, only subpopulations of tumour cells exhibited this rearrangement indicating genetic heterogeneity.
The comparison of visual and automated scoring of FISH signals revealed concordant results in 19 out of 22 cases (87%) indicating reliable scoring results using the optimised scoring parameter for RET/PTC with the automated Metafer4 system.
It can be concluded from this study that genomic rearrangements are frequent in PTC and therefore important events in thyroid carcinogenesis.
American Psychological Association (APA)
Hieber, Ludwig& Huber, Reinhard& Bauer, Verena& Schäffner, Quirin& Braselmann, Herbert& Thomas, Geraldine…[et al.]. 2011. Chromosomal Rearrangements in Post-Chernobyl Papillary Thyroid Carcinomas: Evaluation by Spectral Karyotyping and Automated Interphase FISH. BioMed Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990250
Modern Language Association (MLA)
Hieber, Ludwig…[et al.]. Chromosomal Rearrangements in Post-Chernobyl Papillary Thyroid Carcinomas: Evaluation by Spectral Karyotyping and Automated Interphase FISH. BioMed Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-990250
American Medical Association (AMA)
Hieber, Ludwig& Huber, Reinhard& Bauer, Verena& Schäffner, Quirin& Braselmann, Herbert& Thomas, Geraldine…[et al.]. Chromosomal Rearrangements in Post-Chernobyl Papillary Thyroid Carcinomas: Evaluation by Spectral Karyotyping and Automated Interphase FISH. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990250
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990250