Proteomics Analyses of the Opportunistic Pathogen Burkholderia vietnamiensis Using Protein Fractionations and Mass Spectrometry
Joint Authors
Wickramasekara, Samanthi
Wysocki, Vicki
Neilson, Julie
Breci, Linda
Maier, Raina M.
Patel, Naren
Hilderbrand, Amy
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The main objectives of this work were to obtain a more extensive coverage of the Burkholderia vietnamiensis proteome than previously reported and to identify virulence factors using tandem mass spectrometry.
The proteome of B.
vietnamiensis was precipitated into four fractions to as extracellular, intracellular, cell surface and cell wall proteins.
Two different approaches were used to analyze the proteins.
The first was a gel-based method where 1D SDS-PAGE was used for separation of the proteins prior to reverse phase liquid chromatography tandem mass spectrometry (LC-MS/MS).
The second method used MudPIT analysis (Multi dimensional Protein Identification Technique), where proteins are digested and separated using cation exchange and reversed phase separations before the MS/MS analysis (LC/LC-MS/MS).
Overall, gel-based LC-MS/MS analysis resulted in more protein identifications than the MudPIT analysis.
Combination of the results lead to identification of more than 1200 proteins, approximately 16% of the proteins coded from the annotated genome of Burkholderia species.
Several virulence factors were detected including flagellin, porin, peroxiredoxin and zinc proteases.
American Psychological Association (APA)
Wickramasekara, Samanthi& Neilson, Julie& Patel, Naren& Breci, Linda& Hilderbrand, Amy& Maier, Raina M.…[et al.]. 2011. Proteomics Analyses of the Opportunistic Pathogen Burkholderia vietnamiensis Using Protein Fractionations and Mass Spectrometry. BioMed Research International،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-990252
Modern Language Association (MLA)
Wickramasekara, Samanthi…[et al.]. Proteomics Analyses of the Opportunistic Pathogen Burkholderia vietnamiensis Using Protein Fractionations and Mass Spectrometry. BioMed Research International No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-990252
American Medical Association (AMA)
Wickramasekara, Samanthi& Neilson, Julie& Patel, Naren& Breci, Linda& Hilderbrand, Amy& Maier, Raina M.…[et al.]. Proteomics Analyses of the Opportunistic Pathogen Burkholderia vietnamiensis Using Protein Fractionations and Mass Spectrometry. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-990252
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990252